RSS 1.0Griffith Research Online
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Recent Submissions
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Parents' Experiences of Suicide-Bereavement: A Qualitative Study at 6 and 12 Months after Loss
(International Journal of Environmental Research and Public Health, 2018)The death of a child by suicide is a severe trauma, placing parents at greater risk of psychological morbidity and physical health problems compared to other causes of death. However, few studies have examined the aftermath ...JOURNAL ARTICLE -
Biochemical properties and substrate specificities of a recombinantly produced Azotobacter vinelandii alginate lyase
(Journal of Bacteriology, 1998)Alginate is a polysaccharide composed of β-D-mannuronic acid (M) and α-L-guluronic acid (G). An Azotobacter vinelandii alginate lyase gene,algL, was cloned, sequenced, and expressed inEscherichia coli. The deduced molecular ...JOURNAL ARTICLE -
''Pushed beyond my comfort zone'': MBA student experiences of conducting qualitative research
(Academy of Management Learning & Education, 2017)Despite the extensive use of qualitative research methods in the management field, there has been little empirical attention paid to how business and management students learn qualitative research skills. This research ...JOURNAL ARTICLE -
Analysis of 4-Phosphopantetheinylation of Polyhydroxybutyrate Synthase from Ralstonia eutropha: Generation of β-Alanine Auxotrophic Tn5 Mutants and Cloning of the panD Gene Region
(Journal of Bacteriology, 1999)The postulated posttranslational modification of the polyhydroxybutyrate (PHA) synthase from Ralstonia eutrophaby 4-phosphopantetheine was investigated. Four β-alanine auxotrophic Tn5-induced mutants of R. eutropha HF39 ...JOURNAL ARTICLE -
A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds
(Archives of Microbiology, 1999)The oxazine dye Nile blue A and its fluorescent oxazone form, Nile red, were used to develop a simple and highly sensitive staining method to detect poly(3-hydroxybutyric acid) and other polyhydroxyalkanoic acids (PHAs) ...JOURNAL ARTICLE -
Analysis of in vivo substrate specificity of the PHA synthase from Ralstonia eutropha: Formation of novel copolyesters in recombinant Escherichia coli
(FEMS Microbiology Letters, 2000)In order to investigate the in vivo substrate specificity of the type I polyhydroxyalkanoate (PHA) synthase from Ralstonia eutropha, we functionally expressed the PHA synthase gene in various Escherichia coli mutants ...JOURNAL ARTICLE -
The Pseudomonas aeruginosa phaG gene product is involved in the synthesis of polyhydroxyalkanoic acid consisting of medium-chain-length constituents from non-related carbon sources
(FEMS Microbiology Letters, 2000)We recently identified the phaGPp gene encoding (R)-3-hydroxydecanoyl-ACP:CoA transacylase in Pseudomonas putida, which directly links the fatty acid de novo biosynthesis and polyhydroxyalkanoate (PHA) biosynthesis. An ...JOURNAL ARTICLE -
In vitro synthesis of poly(3-hydroxydecanoate): Purification and enzymatic characterization of type II polyhydroxyalkanoate synthases PhaC1 and PhaC2 from Pseudomonas aeruginosa
(Applied Microbiology and Biotechnology, 2000)For the first time, the purification has been achieved of the type II polyhydroxyalkanoate (PHA) synthases PhaC1 and PhaC2 from Pseudomonas aeruginosa applying N-terminal His6-tag fusions and metal chelate affinity ...JOURNAL ARTICLE -
Polymer production by two newly isolated extremely halophilic archaea: Application of a novel corrosion-resistant bioreactor
(Applied Microbiology and Biotechnology, 2000)A novel corrosion-resistant bioreactor composed of polyetherether ketone (PEEK), tech glass and silicium nitrite ceramics was constructed and applied for the cultivation of two newly isolated, extremely halophilic archaea ...JOURNAL ARTICLE -
Homologous functional expression of cryptic phaG from Pseudomonas oleovorans establishes the transacylase-mediated polyhydroxyalkanoate biosynthetic pathway
(Applied Microbiology and Biotechnology, 2000)Various pseudomonads are capable of the synthesis of polyhydroxyalkanoate (PHA), composed of medium chain length (MCL) 3-hydroxy fatty acids (C6—C14), when grown on simple carbon sources such as, for example, gluconate or ...JOURNAL ARTICLE -
Polyhydroxybutyrate biosynthesis in Caulobacter crescentus: Molecular characterization of the polyhydroxybutyrate synthase
(Microbiology, 2001)Caulobacter crescentus was investigated with respect to polyhydroxybutyrate (PHB) biosynthesis. Polyhydroxyalkanoate (PHA) accumulation contributing to approximately 18% of the cell dry weight was obtained in the presence ...JOURNAL ARTICLE -
In vivo evolution of the Aeromonas punctata polyhydroxyalkanoate (PHA) synthase: Isolation and characterization of modified PHA synthases with enhanced activity
(Applied Microbiology and Biotechnology, 2002)In vivo random mutagenesis of the polyhydroxyalkanoate (PHA) synthase gene from Aeromonas punctata was performed employing the mutator strain Escherichia coli XL1-Red. About 200,000 mutants were screened on Nile red-containing ...JOURNAL ARTICLE -
Formation of short chain length/medium chain length polyhydroxyalkanoate copolymers by fatty acid b-oxidation inhibited Ralstonia eutropha
(Biomacromolecules, 2002)Ralstonia eutropha has been considered as a bacterium, incorporating hydroxyalkanoates of less than six carbons only into polyhydroxyalkanoates (PHAs). Cells of the wild type cultivated with sodium octanoate as the carbon ...JOURNAL ARTICLE -
Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
(Applied and Environmental Microbiology, 2001)Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and ...JOURNAL ARTICLE -
Metabolic routing towards polyhydroxyalkanoic acid synthesis in recombinant Escherichia coli (fadR): Inhibition of fatty acid β-oxidation by acrylic acid
(FEMS Microbiology Letters, 1998)Heterologous expression of the phaC1 gene from Pseudomonas aeruginosa, which encodes one of the polyhydroxyalkanoic acid synthases, in Escherichia coli impaired in fatty acid β-oxidation results in polyhydroxyalkanoic acid ...JOURNAL ARTICLE -
A new metabolic link between fatty acid de novo synthesis and polyhydroxyalkanoic acid synthesis. The phaG gene from Pseudomonas putida KT2440 encodes a 3-hydroxyacyl-acyl carrier protein-coenzyme a transferase
(Journal of Biological Chemistry, 1998)To investigate the metabolic link between fatty acid de novo synthesis and polyhydroxyalkanoic acid (PHA) synthesis, we isolated mutants of Pseudomonas putida KT2440 deficient in this metabolic route. The gene phaG was ...JOURNAL ARTICLE -
Transfer of Natrialba asiatica B1T to Natrialba taiwanensis sp. nov. and description of Natrialba aegyptiaca sp. nov., a novel extremely halophilic, aerobic, non-pigmented member of the Archaea from Egypt that produces extracellular poly(glutamic acid)
(International Journal of Systematic and Evolutionary Microbiology, 2001)A novel extremely halophilic member of the Archaea, strain 40T, was isolated from Egypt (Aswan). This isolate requires at least 1.6 M sodium chloride for growth and exhibits optimal growth between 37 and 42 degrees C. ...JOURNAL ARTICLE -
Bioinformatic tools for DNA/protein sequence analysis, functional assignment of genes and protein classification
(Applied Microbiology and Biotechnology, 2001)The development of efficient DNA sequencing methods has led to the achievement of the DNA sequence of entire genomes from (to date) 55 prokaryotes, 5 eukaryotic organisms and 10 eukaryotic chromosomes. Thus, an enormous ...JOURNAL ARTICLE -
Heterologous expression of the acyl-acyl carrier protein thioesterase gene from the plant Umbellularia californica mediates polyhydroxyalkanoate biosynthesis in recombinant Escherichia coli
(Applied Microbiology and Biotechnology, 2001)The acyl–acyl carrier protein (ACP) thioesterase cDNA from the plant Umbellularia californica was functionally expressed in various recombinant Escherichia coli strains in order to establish a new metabolic route toward ...JOURNAL ARTICLE -
Molecular characterization of the poly(3-hydroxybutyrate) (PHB) synthase from Ralstonia eutropha: In vitro evolution, site-specific mutagenesis and development of a PHB synthase protein model
(Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, 2002)A threading model of the Ralstonia eutropha polyhydroxyalkanoate (PHA) synthase was developed based on the homology to the Burkholderia glumae lipase, whose structure has been resolved by X-ray analysis. The lid-like ...JOURNAL ARTICLE