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dc.contributor.authorHou, Xiaotao
dc.contributor.authorDeng, Jiagang
dc.contributor.authorZhang, Qian
dc.contributor.authorWang, Dongdong
dc.contributor.authorKennedy, Derek
dc.contributor.authorQuinn, Ronald J
dc.contributor.authorFeng, Yunjiang
dc.date.accessioned2017-11-28T03:56:10Z
dc.date.available2017-11-28T03:56:10Z
dc.date.issued2016
dc.identifier.issn0378-8741
dc.identifier.doi10.1016/j.jep.2016.09.016
dc.identifier.urihttp://hdl.handle.net/10072/100240
dc.description.abstractEthnopharmacological relevance The ethnic Chinese Yao medicine Baizuan, which are the leaves of Schisandra viridis A. C. Smith, is traditionally used, in combination with other herbs, to soften hard lumps and dispel nodes in the treatment of cancer, however, this property has not been well studied with a clear indication of the active principles. Aim of the study The experiments were carried out to investigate the cytotoxic activity of the extracts and to identify the active principles from the extract, which could support the traditional application of treating cancer. Materials and methods Dried and ground plant material was extracted with water and ethanol and further purified by HPLC. The cytotoxicity of the extracts, fractions and pure compounds were evaluated for their abilities to inhibit the proliferation of breast cancer cells MCF7 and tongue cancer cells CAL27. The cytotoxicity of the pure compounds were also tested against Human Embryonic Kidney cell line HEK293. Results Both aqueous and ethanol extracts showed activities against MCF7 and CAL27 cancer cells. Bioassay-guided fractionation and purification of the extracts resulted in six active principles, including five dibenzocyclooctene lignans namely gomisin H (1), schisandrin (2), angeloylgomisin H (3), (+)-gomisin M2 (4) and (-)-rubschisandrin (5), and one triterpenoid, schisanol (6). Compounds 1-3 showed moderate cytotoxic activities with IC50 values ranging from 100 to 200 µg/mL against MCF7 and CAL27 cell lines. Dioxane containing lignans 4-5 and triterpenoid 6 were 10 times more active with IC50 values of 14.5, 13.4, 10.6 µg/mL against MCF7, and 21.2, 17.9, 11.7 µg/mL against CAL27, respectively. Compounds 1-6 also showed cytotoxicity against HEK293 with IC50 values ranging from 10 to 150 µg/mL, respectively. Conclusions The traditional extraction protocol using boiled water afforded three moderately active lignans 1-3. Ethanol extraction, which is widely used in the preparation of herbal remedies in China, yielded three additional active compounds 4-5 with more potent activities. These results provided a rationale for the traditional application of the ethnic Yao medicine Baizuan in the treatment of cancer.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom146
dc.relation.ispartofpageto152
dc.relation.ispartofjournalJournal of Ethnopharmacology
dc.relation.ispartofvolume194
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences not elsewhere classified
dc.subject.fieldofresearchPlant Biology
dc.subject.fieldofresearchComplementary and Alternative Medicine
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.fieldofresearchcode111599
dc.subject.fieldofresearchcode0607
dc.subject.fieldofresearchcode1104
dc.subject.fieldofresearchcode1115
dc.titleCytotoxic ethnic Yao medicine Baizuan, leaves of Schisandra viridis A. C. Smith
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Sciences, Griffith Institute for Drug Discovery
gro.hasfulltextNo Full Text
gro.griffith.authorKennedy, Derek D.
gro.griffith.authorQuinn, Ronald J.
gro.griffith.authorFeng, Yun J.
gro.griffith.authorWang, Dongdong
gro.griffith.authorZhang, Qian
gro.griffith.authorHou, Xiaotao


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