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dc.contributor.authorWang, Tong
dc.contributor.authorMartin, Sally
dc.contributor.authorNguyen, Tam H
dc.contributor.authorHarper, Callista B
dc.contributor.authorGormal, Rachel S
dc.contributor.authorMartinez-Marmol, Ramon
dc.contributor.authorKarunanithi, Shanker
dc.contributor.authorCoulson, Elizabeth J
dc.contributor.authorGlass, Nick R
dc.contributor.authorCooper-White, Justin J
dc.contributor.authorvan Swinderen, Bruno
dc.contributor.authorMeunier, Frederic A
dc.date.accessioned2018-01-18T03:50:22Z
dc.date.available2018-01-18T03:50:22Z
dc.date.issued2016
dc.identifier.issn2041-1723
dc.identifier.doi10.1038/ncomms12976
dc.identifier.urihttp://hdl.handle.net/10072/100488
dc.description.abstractAxonal retrograde transport of signalling endosomes from the nerve terminal to the soma underpins survival. As each signalling endosome carries a quantal amount of activated receptors, we hypothesized that it is the frequency of endosomes reaching the soma that determines the scale of the trophic signal. Here we show that upregulating synaptic activity markedly increased the flux of plasma membrane-derived retrograde endosomes (labelled using cholera toxin subunit-B: CTB) in hippocampal neurons cultured in microfluidic devices, and live Drosophila larval motor neurons. Electron and super-resolution microscopy analyses revealed that the fast-moving sub-diffraction-limited CTB carriers contained the TrkB neurotrophin receptor, transiently activated by synaptic activity in a BDNF-independent manner. Pharmacological and genetic inhibition of TrkB activation selectively prevented the coupling between synaptic activity and the retrograde flux of signalling endosomes. TrkB activity therefore controls the encoding of synaptic activity experienced by nerve terminals, digitalized as the flux of retrogradely transported signalling endosomes.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofpagefrom12976-1
dc.relation.ispartofpageto12976-15
dc.relation.ispartofjournalNature Communications
dc.relation.ispartofvolume7
dc.subject.fieldofresearchNeurosciences not elsewhere classified
dc.subject.fieldofresearchcode320999
dc.titleFlux of signalling endosomes undergoing axonal retrograde transport is encoded by presynaptic activity and TrkB
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2016 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
gro.hasfulltextFull Text
gro.griffith.authorKarunanithi, Shanker
gro.griffith.authorGormal, Rachel


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