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dc.contributor.authorMenon, Shruti
dc.contributor.authorStansfield, Scott H
dc.contributor.authorLogan, Benignus
dc.contributor.authorHocking, Jane S
dc.contributor.authorTimms, Peter
dc.contributor.authorRombauts, Luk
dc.contributor.authorAllan, John A
dc.contributor.authorHuston, Wilhelmina M
dc.date.accessioned2019-03-28T23:05:28Z
dc.date.available2019-03-28T23:05:28Z
dc.date.issued2016
dc.identifier.issn0022-2615
dc.identifier.doi10.1099/jmm.0.000311
dc.identifier.urihttp://hdl.handle.net/10072/100518
dc.description.abstractChlamydia trachomatis results in tubal factor infertility in some women. Diagnosis of this tubal infertility is difficult and typically involves laparoscopy or hysterosalpingography to detect the tubal blockages. Numerous serological tests have been developed; however, they are presently not used for diagnosis without subsequent surgical investigation during the infertility investigation. This study aimed to develop a highly specific serological assay for chlamydial tubal factor infertility in women that could be used to recommend direct progression to in vitro fertilization (IVF) treatment for women who are positive. Women were recruited from a variety of settings including women seeking fertility treatment, sexual health and general practitioner (GP) consultations or antenatal care (n=259). The serological assay was developed using sera from a large group of women by using infertile microimmunofluorescence (MIF)-positive women with tubal damage as the positives compared to infertile or acute infection and/or fertile controls (negatives). The new multi-peptide ELISA was highly specific for the detection of tubal factor infertility (P=0.011) compared to another ELISA (P=0.022) and MIF (P=0.099). The sensitivity of the assay should be improved before clinical utility. Potentially, a two-step testing protocol could be used during the initial infertility investigation, where MIF followed by a highly specific ELISA could be used to recommend direct progression to IVF for women who are positive.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherThe Pathological Society of Great Britain and Ireland
dc.relation.ispartofpagefrom915
dc.relation.ispartofpageto922
dc.relation.ispartofjournalJournal of Medical Microbiology
dc.relation.ispartofvolume65
dc.subject.fieldofresearchMedical and Health Sciences not elsewhere classified
dc.subject.fieldofresearchAgricultural and Veterinary Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchcode119999
dc.subject.fieldofresearchcode07
dc.subject.fieldofresearchcode11
dc.subject.fieldofresearchcode06
dc.titleDevelopment and evaluation of a multi-antigen peptide ELISA for the diagnosis of chlamydia trachomatis-related infertility in women
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorLogan, Benignus


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