dc.contributor.author | Abu Choudhury, Md | |
dc.contributor.author | Marsh, Nicole | |
dc.contributor.author | Banu, Shahera | |
dc.contributor.author | Paterson, David L | |
dc.contributor.author | Rickard, Claire M | |
dc.contributor.author | McMillan, David J | |
dc.date.accessioned | 2018-01-19T05:26:45Z | |
dc.date.available | 2018-01-19T05:26:45Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.doi | 10.1371/journal.pone.0146354 | |
dc.identifier.uri | http://hdl.handle.net/10072/100592 | |
dc.description.abstract | Skin bacteria at peripheral intravenous catheter (PIVC) insertion sites pose a serious risk of
microbial migration and subsequent colonisation of PIVCs, and the development of catheter
related bloodstream infections (CRBSIs). Common skin bacteria are often associated with
CRBSIs, therefore the bacterial communities at PIVC skin sites are likely to have major
implications for PIVC colonisation. This study aimed to determine the bacterial community
structures on skin at PIVC insertion sites and to compare the diversity with associated
PIVCs. A total of 10 PIVC skin site swabs and matching PIVC tips were collected by a
research nurse from 10 hospitalised medical/surgical patients at catheter removal. All
swabs and PIVCs underwent traditional culture and high-throughput sequencing. The bacterial
communities on PIVC skin swabs and matching PIVCs were diverse and significantly
associated (correlation coefficient = 0.7, p<0.001). Methylobacterium spp. was the dominant
genus in all PIVC tip samples, but not so for skin swabs. Sixty-one percent of all reads
from the PIVC tips and 36% of all reads from the skin swabs belonged to this genus. Staphylococcus
spp., (26%), Pseudomonas spp., (10%) and Acinetobacter spp. (10%) were
detected from skin swabs but not from PIVC tips. Most skin associated bacteria commonly
associated with CRBSIs were observed on skin sites, but not on PIVCs. Diverse bacterial
communities were observed at skin sites despite skin decolonization at PIVC insertion. The
positive association of skin and PIVC tip communities provides further evidence that skin is
a major source of PIVC colonisation via bacterial migration but microbes present may be different
to those traditionally identified via culture methods. The results provide new insights
into the colonisation of catheters and potential pathogenesis of bacteria associated with
CRBSI, and may assist in developing new strategies designed to reduce the risk of CRBSI. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Public Library of Sciences | |
dc.relation.ispartofpagefrom | e0146354-1 | |
dc.relation.ispartofpageto | e0146354-12 | |
dc.relation.ispartofissue | 1 | |
dc.relation.ispartofjournal | PLoS One | |
dc.relation.ispartofvolume | 11 | |
dc.subject.fieldofresearch | Clinical sciences not elsewhere classified | |
dc.subject.fieldofresearchcode | 320299 | |
dc.title | Molecular Comparison of Bacterial Communities on Peripheral Intravenous Catheters and Matched Skin Swabs | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
dcterms.license | https://creativecommons.org/licenses/by/4.0/ | |
dc.description.version | Version of Record (VoR) | |
gro.faculty | Griffith Health, School of Nursing and Midwifery | |
gro.rights.copyright | © 2016 Choudhury et al. This is an open
access article distributed under the terms of the
Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any
medium, provided the original author and source are
credited. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | McMillan, David J. | |
gro.griffith.author | Rickard, Claire | |
gro.griffith.author | Marsh, Nicole M. | |
gro.griffith.author | Choudhury, Nahid A. | |