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dc.contributor.authorRcom-H'cheo-Gauthier, Alexandre N
dc.contributor.authorDavis, Amelia
dc.contributor.authorMeedeniya, Adrian CB
dc.contributor.authorPountney, Dean L
dc.date.accessioned2019-03-15T00:27:17Z
dc.date.available2019-03-15T00:27:17Z
dc.date.issued2016
dc.identifier.issn1044-7431
dc.identifier.doi10.1016/j.mcn.2016.10.003
dc.identifier.urihttp://hdl.handle.net/10072/100651
dc.description.abstractα-Synuclein (α-syn) aggregates (Lewy bodies) in Dementia with Lewy Bodies (DLB) may be associated with disturbed calcium homeostasis and oxidative stress. We investigated the interplay between α-syn aggregation, expression of the calbindin-D28k (CB) neuronal calcium-buffering protein and oxidative stress, combining immunofluorescence double labelling and Western analysis, and examining DLB and normal human cases and a unilateral oxidative stress lesion model of α-syn disease (rotenone mouse). DLB cases showed a greater proportion of CB + cells in affected brain regions compared to normal cases with Lewy bodies largely present in CB − neurons and virtually undetected in CB + neurons. The unilateral rotenone-lesioned mouse model showed a greater proportion of CB + cells and α-syn aggregates within the lesioned hemisphere than the control hemisphere, especially proximal to the lesion site, and α-syn inclusions occurred primarily in CB − cells and were almost completely absent in CB + cells. Consistent with the immunofluorescence data, Western analysis showed the total CB level was 25% higher in lesioned compared to control hemisphere in aged animals that are more sensitive to lesion and 20% higher in aged compared to young mice in lesioned hemisphere, but not significantly different between young and aged in the control hemisphere. Taken together, the findings show α-syn aggregation is excluded from CB + neurons, although the increased sensitivity of aged animals to lesion was not related to differential CB expression.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherElsevier
dc.relation.ispartofpagefrom65
dc.relation.ispartofpageto75
dc.relation.ispartofjournalMolecular and Cellular Neuroscience
dc.relation.ispartofvolume77
dc.subject.fieldofresearchNeurosciences not elsewhere classified
dc.subject.fieldofresearchNeurosciences
dc.subject.fieldofresearchCognitive Sciences
dc.subject.fieldofresearchPsychology
dc.subject.fieldofresearchcode110999
dc.subject.fieldofresearchcode1109
dc.subject.fieldofresearchcode1702
dc.subject.fieldofresearchcode1701
dc.titleAlpha-synuclein aggregates are excluded from calbindin-D28k-positive neurons in dementia with Lewy bodies and a unilateral rotenone mouse model
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medical Science
gro.hasfulltextNo Full Text
gro.griffith.authorPountney, Dean L.
gro.griffith.authorDavis, Amelia L.
gro.griffith.authorMeedeniya, Adrian C.
gro.griffith.authorRcom-H'cheo-Gauthier, Alexandre N.


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