dc.contributor.author | Rcom-H'cheo-Gauthier, Alexandre N | |
dc.contributor.author | Davis, Amelia | |
dc.contributor.author | Meedeniya, Adrian CB | |
dc.contributor.author | Pountney, Dean L | |
dc.date.accessioned | 2019-03-15T00:27:17Z | |
dc.date.available | 2019-03-15T00:27:17Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 1044-7431 | |
dc.identifier.doi | 10.1016/j.mcn.2016.10.003 | |
dc.identifier.uri | http://hdl.handle.net/10072/100651 | |
dc.description.abstract | α-Synuclein (α-syn) aggregates (Lewy bodies) in Dementia with Lewy Bodies (DLB) may be associated with disturbed calcium homeostasis and oxidative stress. We investigated the interplay between α-syn aggregation, expression of the calbindin-D28k (CB) neuronal calcium-buffering protein and oxidative stress, combining immunofluorescence double labelling and Western analysis, and examining DLB and normal human cases and a unilateral oxidative stress lesion model of α-syn disease (rotenone mouse). DLB cases showed a greater proportion of CB + cells in affected brain regions compared to normal cases with Lewy bodies largely present in CB − neurons and virtually undetected in CB + neurons. The unilateral rotenone-lesioned mouse model showed a greater proportion of CB + cells and α-syn aggregates within the lesioned hemisphere than the control hemisphere, especially proximal to the lesion site, and α-syn inclusions occurred primarily in CB − cells and were almost completely absent in CB + cells. Consistent with the immunofluorescence data, Western analysis showed the total CB level was 25% higher in lesioned compared to control hemisphere in aged animals that are more sensitive to lesion and 20% higher in aged compared to young mice in lesioned hemisphere, but not significantly different between young and aged in the control hemisphere. Taken together, the findings show α-syn aggregation is excluded from CB + neurons, although the increased sensitivity of aged animals to lesion was not related to differential CB expression. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.relation.ispartofpagefrom | 65 | |
dc.relation.ispartofpageto | 75 | |
dc.relation.ispartofjournal | Molecular and Cellular Neuroscience | |
dc.relation.ispartofvolume | 77 | |
dc.subject.fieldofresearch | Neurosciences | |
dc.subject.fieldofresearch | Neurosciences not elsewhere classified | |
dc.subject.fieldofresearch | Psychology | |
dc.subject.fieldofresearch | Cognitive and computational psychology | |
dc.subject.fieldofresearchcode | 3209 | |
dc.subject.fieldofresearchcode | 320999 | |
dc.subject.fieldofresearchcode | 52 | |
dc.subject.fieldofresearchcode | 5204 | |
dc.title | Alpha-synuclein aggregates are excluded from calbindin-D28k-positive neurons in dementia with Lewy bodies and a unilateral rotenone mouse model | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.faculty | Griffith Health, School of Medical Science | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Pountney, Dean L. | |