Protein crystal screening and characterization for serial femtosecond nanocrystallography
Abstract
The recent development of X-ray free electron lasers (XFELs) has spurred the development of serial
femtosecond nanocrystallography (SFX) which, for the first time, is enabling structure retrieval from
sub-micron protein crystals. Although there are already a growing number of structures published
using SFX, the technology is still very new and presents a number of unique challenges as well as
opportunities for structural biologists. One of the biggest barriers to the success of SFX experiments
is the preparation and selection of suitable protein crystal samples. Here we outline a protocol for
preparing and screening for suitable XFEL targets.
Journal Title
Scientific Reports
Volume
6
Copyright Statement
© The Author(s). 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images
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Subject
Biochemistry and Cell Biology not elsewhere classified