A direct-sensing galactose chemoreceptor recently evolved in invasive strains of Campylobacter jejuni

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Author(s)
Day, Christopher J
King, Rebecca M
Shewell, Lucy K
Tram, Greg
Najnin, Tahria
Hartley-Tassell, Lauren E
Wilson, Jennifer C
Fleetwood, Aaron D
Zhulin, Igor B
Korolik, Victoria
Griffith University Author(s)
Year published
2016
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A rare chemotaxis receptor, Tlp11, has been previously identified in invasive strains of
Campylobacter jejuni, the most prevalent cause of bacterial gastroenteritis worldwide. Here we
use glycan and small-molecule arrays, as well as surface plasmon resonance, to show that
Tlp11 specifically interacts with galactose. Tlp11 is required for the chemotactic response of
C. jejuni to galactose, as shown using wild type, allelic inactivation and addition mutants. The
inactivated mutant displays reduced virulence in vivo, in a model of chicken colonization. The
Tlp11 sensory domain represents the first known sugar-binding dCache_1 ...
View more >A rare chemotaxis receptor, Tlp11, has been previously identified in invasive strains of Campylobacter jejuni, the most prevalent cause of bacterial gastroenteritis worldwide. Here we use glycan and small-molecule arrays, as well as surface plasmon resonance, to show that Tlp11 specifically interacts with galactose. Tlp11 is required for the chemotactic response of C. jejuni to galactose, as shown using wild type, allelic inactivation and addition mutants. The inactivated mutant displays reduced virulence in vivo, in a model of chicken colonization. The Tlp11 sensory domain represents the first known sugar-binding dCache_1 domain, which is the most abundant family of extracellular sensors in bacteria. The Tlp11 signalling domain interacts with the chemotaxis scaffolding proteins CheV and CheW, and comparative genomic analysis indicates a likely recent evolutionary origin for Tlp11. We propose to rename Tlp11 as CcrG, Campylobacter ChemoReceptor for Galactose.
View less >
View more >A rare chemotaxis receptor, Tlp11, has been previously identified in invasive strains of Campylobacter jejuni, the most prevalent cause of bacterial gastroenteritis worldwide. Here we use glycan and small-molecule arrays, as well as surface plasmon resonance, to show that Tlp11 specifically interacts with galactose. Tlp11 is required for the chemotactic response of C. jejuni to galactose, as shown using wild type, allelic inactivation and addition mutants. The inactivated mutant displays reduced virulence in vivo, in a model of chicken colonization. The Tlp11 sensory domain represents the first known sugar-binding dCache_1 domain, which is the most abundant family of extracellular sensors in bacteria. The Tlp11 signalling domain interacts with the chemotaxis scaffolding proteins CheV and CheW, and comparative genomic analysis indicates a likely recent evolutionary origin for Tlp11. We propose to rename Tlp11 as CcrG, Campylobacter ChemoReceptor for Galactose.
View less >
Journal Title
Nature Communications
Volume
7
Copyright Statement
© The Author(s) 2016 This work is licensed under a Creative Commons Attribution 4.0
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Subject
Bacteriology