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dc.contributor.authorSakiragaoglu, O
dc.contributor.authorMunn, AL
dc.date.accessioned2017-12-12T05:39:12Z
dc.date.available2017-12-12T05:39:12Z
dc.date.issued2016
dc.identifier.issn1073-6085
dc.identifier.doi10.1007/s12033-016-9982-6
dc.identifier.urihttp://hdl.handle.net/10072/100705
dc.description.abstractTo inhibit telomerase activity, a construct which contains artificial introns in the enhanced green fluorescent protein (EGFP) gene that encodes small hairpin RNA (shRNA) sequences that target human telomerase reverse transcriptase (hTERT) gene expression was designed and tested for its effect on lung cancer cell line. On intron splicing from the construct, intronic sequences were released and formed shRNA in the cells. After transfection of the construct, hTERT mRNA expression decreased by approximately 55 % in A549 cells. Correspondingly, in the same cell line, telomerase activity was decreased by approximately 23 %. The telomerase activity was transiently inhibited by this non-viral shRNA expression system that uses intron splicing to release artificial introns in an EGFP marker gene that contain shRNA targeting telomerase.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofpagefrom832
dc.relation.ispartofpageto837
dc.relation.ispartofissue12
dc.relation.ispartofjournalMolecular Biotechnology
dc.relation.ispartofvolume58
dc.subject.fieldofresearchBiological Sciences not elsewhere classified
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchTechnology
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode069999
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode10
dc.subject.fieldofresearchcode11
dc.titleInhibition of Telomerase Activity Using an EGFP-Intron Splicing System Encoding Multiple RNAi Sequences
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medical Science
gro.hasfulltextNo Full Text
gro.griffith.authorMunn, Alan L.
gro.griffith.authorSakiragaoglu, Onur


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