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dc.contributor.authorPasdar, Elham Alizadeh
dc.contributor.authorSmits, Michael
dc.contributor.authorStapelberg, Michael
dc.contributor.authorBajzikova, Martina
dc.contributor.authorStantic, Marina
dc.contributor.authorGoodwin, Jacob
dc.contributor.authorYan, Bing
dc.contributor.authorStursa, Jan
dc.contributor.authorKovarova, Jaromira
dc.contributor.authorSachaphibulkij, Karishma
dc.contributor.authorBezawork-Geleta, Ayenachew
dc.contributor.authorSobol, Margaryta
dc.contributor.authorFilimonenko, Anatoly
dc.contributor.authorTomasetti, Marco
dc.contributor.authorZobalova, Renata
dc.contributor.authorHozak, Pavel
dc.contributor.authorDong, Lan-Feng
dc.contributor.authorNeuzil, Jiri
dc.date.accessioned2017-05-03T12:35:41Z
dc.date.available2017-05-03T12:35:41Z
dc.date.issued2015
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0119549
dc.identifier.urihttp://hdl.handle.net/10072/100912
dc.description.abstractMalignant mesothelioma (MM) is an aggressive type of tumour causing high mortality. One reason for this paradigm may be the existence of a subpopulation of tumour-initiating cells (TICs) that endow MM with drug resistance and recurrence. The objective of this study was to identify and characterise a TIC subpopulation in MM cells, using spheroid cultures, mesospheres, as a model of MM TICs. Mesospheres, typified by the stemness markers CD24, ABCG2 and OCT4, initiated tumours in immunodeficient mice more efficiently than adherent cells. CD24 knock-down cells lost the sphere-forming capacity and featured lower tumorigenicity. Upon serial transplantation, mesospheres were gradually more efficiently tumrigenic with increased level of stem cell markers. We also show that mesospheres feature mitochondrial and metabolic properties similar to those of normal and cancer stem cells. Finally, we show that mesothelioma-initiating cells are highly susceptible to mitochondrially targeted vitamin E succinate. This study documents that mesospheres can be used as a plausible model of mesothelioma-initiating cells and that they can be utilised in the search for efficient agents against MM.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherPublic Library of Sciences
dc.relation.ispartofpagefrome0119549-1
dc.relation.ispartofpagetoe0119549-21
dc.relation.ispartofissue5
dc.relation.ispartofjournalPLoS One
dc.relation.ispartofvolume10
dc.subject.fieldofresearchCancer cell biology
dc.subject.fieldofresearchcode321101
dc.titleCharacterisation of mesothelioma-initiating cells and their susceptibility to anti-cancer agents
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.facultyGriffith Health, School of Medical Science
gro.rights.copyright© 2015 Pasdar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
gro.hasfulltextFull Text
gro.griffith.authorNeuzil, Jiri
gro.griffith.authorStapelberg, Michael
gro.griffith.authorZobalova, Renata
gro.griffith.authorStantic, Marina
gro.griffith.authorDong, Lan-feng
gro.griffith.authorSmits, Michael A.
gro.griffith.authorGoodwin, Jacob
gro.griffith.authorAlizadeh Pasdar, Elham
gro.griffith.authorYan, Bing
gro.griffith.authorSachaphibulkij, Karishma
gro.griffith.authorBezawork-Geleta, Ayenachew


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