dc.contributor.author | Moriel, Danilo G | |
dc.contributor.author | Tan, Lendl | |
dc.contributor.author | Goh, Kelvin GK | |
dc.contributor.author | Minh-Duy, Phan | |
dc.contributor.author | Ipe, Deepak S | |
dc.contributor.author | Lo, Alvin W | |
dc.contributor.author | Peters, Kate M | |
dc.contributor.author | Ulett, Glen C | |
dc.contributor.author | Beatson, Scott A | |
dc.contributor.author | Schembri, Mark A | |
dc.date.accessioned | 2018-01-19T01:13:14Z | |
dc.date.available | 2018-01-19T01:13:14Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 2379-5042 | |
dc.identifier.doi | 10.1128/mSphere.00326-16 | |
dc.identifier.uri | http://hdl.handle.net/10072/100916 | |
dc.description.abstract | Escherichia coli is a versatile pathogen capable of causing intestinal
and extraintestinal infections that result in a huge burden of global human disease.
The diversity of E. coli is reflected by its multiple different pathotypes and mosaic
genome composition. E. coli strains are also a major driver of antibiotic resistance,
emphasizing the urgent need for new treatment and prevention measures. Here, we
used a large data set comprising 1,700 draft and complete genomes to define the
core and accessory genome of E. coli and demonstrated the overlapping relationship
between strains from different pathotypes. In combination with proteomic investigation,
this analysis revealed core genes that encode surface-exposed or secreted proteins
that represent potential broad-coverage vaccine antigens. One of these antigens,
YncE, was characterized as a conserved immunogenic antigen able to protect
against acute systemic infection in mice after vaccination. Overall, this work provides
a genomic blueprint for future analyses of conserved and accessory E. coli genes.
The work also identified YncE as a novel antigen that could be exploited in the development
of a vaccine against all pathogenic E. coli strains—an important direction
given the high global incidence of infections caused by multidrug-resistant strains
for which there are few effective antibiotics. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | American Society for Microbiology | |
dc.relation.ispartofpagefrom | e00326-1 | |
dc.relation.ispartofpageto | e00326-13 | |
dc.relation.ispartofissue | 6 | |
dc.relation.ispartofjournal | mSphere | |
dc.relation.ispartofvolume | 1 | |
dc.subject.fieldofresearch | Medical bacteriology | |
dc.subject.fieldofresearchcode | 320701 | |
dc.title | A Novel Protective Vaccine Antigen from the Core Escherichia coli Genome | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
dcterms.license | https://creativecommons.org/licenses/by/4.0/ | |
dc.description.version | Version of Record (VoR) | |
gro.rights.copyright | © 2016 Moriel et al. This is an open access
article distributed under the terms of
the Creative Commons Attribution 4.0
International license. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Ulett, Glen C. | |