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dc.contributor.authorSteenhauer, Lisa M
dc.contributor.authorWierenga, Joren
dc.contributor.authorCarreira, Catia
dc.contributor.authorLimpens, Ronald WAL
dc.contributor.authorKoster, Abraham J
dc.contributor.authorPollard, Peter C
dc.contributor.authorBrussaard, Corina PD
dc.date.accessioned2019-03-15T06:34:48Z
dc.date.available2019-03-15T06:34:48Z
dc.date.issued2016
dc.identifier.issn0948-3055
dc.identifier.doi10.3354/ame01800
dc.identifier.urihttp://hdl.handle.net/10072/101135
dc.description.abstractA lytic virus that infects a European strain of the freshwater filamentous cyano-bacterium Cylindrospermopsis raciborskii was isolated from a lake in the Netherlands and partially characterised. With a genome size of 110 ± 15 kb, an icosahedral capsid of 65 ± 1 nm (n = 22) and a long non-contractile tail of 612 ± 31 nm (n = 15), this dsDNA cyanophage CrV appears to belong to the Siphoviridae family. CrV was highly host specific, not infecting other filamentous cyano - bacteria species isolated from the same lake, nor 4 Australian strains of C. raciborskii. The latent period of this cyanophage was 20−24 h. Varying the irradiance affected cyanophage−host inter - actions: at low light (20 µmol quanta m−2 s−1) the latent period was 1.3 times longer compared with at mid light (90 µmol quanta m−2 s−1); burst size at mid light was 332 CrV per lysed host cell, at low light it was halved (48%) and at high light (250 µmol quanta m−2 s−1) the burst size was further reduced to only 14% of that of mid light. Temperature also affected the virus growth characteristics: the CrV latent period at high temperature (30°C) was reduced to just 11% (compared with a mid temperature of 22°C), but still the burst size increased to 541 CrV per lysed host cell; at low temperature (15°C) the latent period was prolonged 1.3-fold and the burst size was reduced to 43%. Our findings indicate that ecologically relevant environmental factors can affect the extent of viral lysis of C. raciborskii, advancing our understanding of the spread of this invasive cyano bacterium across Europe.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherInter Research
dc.relation.ispartofpagefrom11
dc.relation.ispartofpageto23
dc.relation.ispartofjournalAquatic Microbial Ecology
dc.relation.ispartofvolume78
dc.subject.fieldofresearchEcology
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchMicrobiology not elsewhere classified
dc.subject.fieldofresearchcode3103
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode310799
dc.subject.keywordsInvasive cyanobacteria
dc.subject.keywordsCyanophage
dc.subject.keywordsFreshwater
dc.subject.keywordsLatent period
dc.subject.keywordsBurst size
dc.subject.keywordsTemperature
dc.subject.keywordsLight
dc.titleIsolation of cyanophage CrV infecting Cylindrospermopsis raciborskii and the influence of temperature and irradiance on CrV proliferation
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Sciences, Griffith School of Environment
gro.rights.copyright© 2016 Inter-Research. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.hasfulltextFull Text
gro.griffith.authorPollard, Peter C.
gro.griffith.authorSteenhauer, Lisa M.


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