dc.contributor.author | Steenhauer, Lisa M | |
dc.contributor.author | Wierenga, Joren | |
dc.contributor.author | Carreira, Catia | |
dc.contributor.author | Limpens, Ronald WAL | |
dc.contributor.author | Koster, Abraham J | |
dc.contributor.author | Pollard, Peter C | |
dc.contributor.author | Brussaard, Corina PD | |
dc.date.accessioned | 2019-03-15T06:34:48Z | |
dc.date.available | 2019-03-15T06:34:48Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0948-3055 | |
dc.identifier.doi | 10.3354/ame01800 | |
dc.identifier.uri | http://hdl.handle.net/10072/101135 | |
dc.description.abstract | A lytic virus that infects a European strain of the freshwater filamentous cyano-bacterium Cylindrospermopsis raciborskii was isolated from a lake in the Netherlands and partially
characterised. With a genome size of 110 ± 15 kb, an icosahedral capsid of 65 ± 1 nm (n = 22) and
a long non-contractile tail of 612 ± 31 nm (n = 15), this dsDNA cyanophage CrV appears to belong
to the Siphoviridae family. CrV was highly host specific, not infecting other filamentous cyano -
bacteria species isolated from the same lake, nor 4 Australian strains of C. raciborskii. The latent
period of this cyanophage was 20−24 h. Varying the irradiance affected cyanophage−host inter -
actions: at low light (20 µmol quanta m−2 s−1) the latent period was 1.3 times longer compared with
at mid light (90 µmol quanta m−2 s−1); burst size at mid light was 332 CrV per lysed host cell, at low
light it was halved (48%) and at high light (250 µmol quanta m−2 s−1) the burst size was further
reduced to only 14% of that of mid light. Temperature also affected the virus growth characteristics: the CrV latent period at high temperature (30°C) was reduced to just 11% (compared with a
mid temperature of 22°C), but still the burst size increased to 541 CrV per lysed host cell; at low
temperature (15°C) the latent period was prolonged 1.3-fold and the burst size was reduced to
43%. Our findings indicate that ecologically relevant environmental factors can affect the extent
of viral lysis of C. raciborskii, advancing our understanding of the spread of this invasive cyano bacterium across Europe. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Inter Research | |
dc.relation.ispartofpagefrom | 11 | |
dc.relation.ispartofpageto | 23 | |
dc.relation.ispartofjournal | Aquatic Microbial Ecology | |
dc.relation.ispartofvolume | 78 | |
dc.subject.fieldofresearch | Ecology | |
dc.subject.fieldofresearch | Microbiology | |
dc.subject.fieldofresearch | Microbiology not elsewhere classified | |
dc.subject.fieldofresearchcode | 3103 | |
dc.subject.fieldofresearchcode | 3107 | |
dc.subject.fieldofresearchcode | 310799 | |
dc.subject.keywords | Invasive cyanobacteria | |
dc.subject.keywords | Cyanophage | |
dc.subject.keywords | Freshwater | |
dc.subject.keywords | Latent period | |
dc.subject.keywords | Burst size | |
dc.subject.keywords | Temperature | |
dc.subject.keywords | Light | |
dc.title | Isolation of cyanophage CrV infecting Cylindrospermopsis raciborskii and the influence of temperature and irradiance on CrV proliferation | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.faculty | Griffith Sciences, Griffith School of Environment | |
gro.rights.copyright | © 2016 Inter-Research. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Pollard, Peter C. | |
gro.griffith.author | Steenhauer, Lisa M. | |