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dc.contributor.authorMasters, N
dc.contributor.authorChristie, M
dc.contributor.authorStratton, H
dc.contributor.authorKatouli, M
dc.date.accessioned2019-03-06T06:56:42Z
dc.date.available2019-03-06T06:56:42Z
dc.date.issued2015
dc.identifier.issn0008-4166
dc.identifier.doi10.1139/cjm-2015-0020
dc.identifier.urihttp://hdl.handle.net/10072/101311
dc.description.abstractWe studied the survival of Escherichia coli and enterococci populations in fecal samples of 7 host species after storage at –20 and –80 °C for 30 days. Composite fecal samples were collected from cows, chickens, horses, pigs, dogs, birds, and humans, and bacteria were enumerated before and after storage. Twenty-eight colonies of each bacterial species were typed before and after storage and the strains were assigned to different biochemical phenotypes (BPTs). A significant reduction in the number of E. coli was observed in all samples stored at –20 °C but in only 3 of those samples stored at –80 °C. However, the numbers of enterococci were similar in most stored samples (except cow and birds). The number and the distribution of E. coli and enterococci BPTs in fresh samples did not vary significantly from those stored at either temperature. Furthermore, the population structure of E. coli and enterococci did not change significantly after storage at –80 °C, this was always the case for those samples stored at –20 °C. We conclude that for those studies investigating E. coli or enterococci population structure, short-term storage (≤30 days) of fecal samples in a glycerol broth at –80 °C is a preferable option.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNRC Research Press
dc.relation.ispartofpagefrom495
dc.relation.ispartofpageto501
dc.relation.ispartofissue7
dc.relation.ispartofjournalCanadian Journal of Microbiology
dc.relation.ispartofvolume61
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchMicrobiology not elsewhere classified
dc.subject.fieldofresearchVeterinary sciences
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode310799
dc.subject.fieldofresearchcode3009
dc.subject.fieldofresearchcode3207
dc.titleViability and stability of escherichia coli and enterococci populations in fecal samples upon freezing
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyright© The Author(s) 2015. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.hasfulltextFull Text
gro.griffith.authorStratton, Helen M.


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