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  • P2X7 receptors mediate innate phagocytosis by human neural precursor cells and neuroblasts

    Author(s)
    Lovelace, Michael D
    Gu, Ben J
    Eamegdool, Steven S
    Weible, Michael W
    Wiley, James S
    Allen, David G
    Chan-Ling, Tailoi
    Griffith University Author(s)
    Weible, Michael W.
    Year published
    2015
    Metadata
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    Abstract
    During early human neurogenesis there is overproduction of neuroblasts and neurons accompanied by widespread programmed cell death (PCD). While it is understood that CD68+ microglia and astrocytes mediate phagocytosis during target‐dependent PCD, little is known of the cell identity or the scavenger molecules used to remove apoptotic corpses during the earliest stages of human neurogenesis. Using a combination of multiple‐marker immunohistochemical staining, functional blocking antibodies and antagonists, we showed that human neural precursor cells (hNPCs) and neuroblasts express functional P2X7 receptors. Furthermore, using ...
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    During early human neurogenesis there is overproduction of neuroblasts and neurons accompanied by widespread programmed cell death (PCD). While it is understood that CD68+ microglia and astrocytes mediate phagocytosis during target‐dependent PCD, little is known of the cell identity or the scavenger molecules used to remove apoptotic corpses during the earliest stages of human neurogenesis. Using a combination of multiple‐marker immunohistochemical staining, functional blocking antibodies and antagonists, we showed that human neural precursor cells (hNPCs) and neuroblasts express functional P2X7 receptors. Furthermore, using live‐cell imaging, flow cytometry, phagocytic assays, and siRNA knockdown, we showed that in a serum‐free environment, doublecortin+ (DCX) neuroblasts and hNPCs can clear apoptotic cells by innate phagocytosis mediated via P2X7. We found that both P2X7highDCXlow hNPCs and P2X7highDCXhigh neuroblasts, derived from primary cultures of human fetal telencephalon, phagocytosed targets including latex beads, apoptotic ReNcells, and apoptotic hNPC/neuroblasts. Pretreatment of neuroblasts and hNPCs with 1 mM adenosine triphosphate (ATP), 100 µM OxATP (P2X7 antagonist), or siRNA knockdown of P2X7 inhibited phagocytosis of these targets. Our results show that P2X7 functions as a scavenger receptor under serum‐free conditions resembling those in early neurogenesis. This is the first demonstration that hNPCs and neuroblasts may participate in clearance of apoptotic corpses during pre target‐dependent neurogenesis and mediate phagocytosis using P2X7 as a scavenger receptor.
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    Journal Title
    Stem Cells
    Volume
    33
    Issue
    2
    DOI
    https://doi.org/10.1002/stem.1864
    Subject
    Biological sciences
    Other biological sciences not elsewhere classified
    Biomedical and clinical sciences
    Publication URI
    http://hdl.handle.net/10072/101325
    Collection
    • Journal articles

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