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dc.contributor.authorYuan, Lin-Ling
dc.contributor.authorChen, Xiulan
dc.contributor.authorZong, Qiong
dc.contributor.authorZhao, Ting
dc.contributor.authorWang, Jia-Lin
dc.contributor.authorZheng, Ya
dc.contributor.authorZhang, Ming
dc.contributor.authorWang, Zailong
dc.contributor.authorBrownlie, Jeremy C
dc.contributor.authorYang, Fuquan
dc.contributor.authorWang, Yu-Feng
dc.date.accessioned2018-11-06T00:59:34Z
dc.date.available2018-11-06T00:59:34Z
dc.date.issued2015
dc.identifier.issn1535-3893
dc.identifier.doi10.1021/acs.jproteome.5b00191
dc.identifier.urihttp://hdl.handle.net/10072/101643
dc.description.abstractTo investigate the molecular mechanisms of cytoplasmic incompatibility (CI) induced by Wolbachia bacteria in Drosophila melanogaster, we applied an isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic assay to identify differentially expressed proteins extracted from spermathecae and seminal receptacles (SSR) of uninfected females mated with either 1-day-old Wolbachia-uninfected (1T) or infected males (1W) or 5-day-old infected males (5W). In total, 1317 proteins were quantified; 83 proteins were identified as having at least a 1.5-fold change in expression when 1W was compared with 1T. Differentially expressed proteins were related to metabolism, immunity, and reproduction. Wolbachia changed the expression of seminal fluid proteins (Sfps). Wolbachia may disrupt the abundance of proteins in SSR by affecting ubiquitin–proteasome-mediated proteolysis. Knocking down two Sfp genes (CG9334 and CG2668) in Wolbachia-free males resulted in significantly lower embryonic hatch rates with a phenotype of chromatin bridges. Wolbachia-infected females may rescue the hatch rates. This suggests that the changed expression of some Sfps may be one of the mechanisms of CI induced by Wolbachia. This study provides a panel of candidate proteins that may be involved in the interaction between Wolbachia and their insect hosts and, through future functional studies, may help to elucidate the underlying mechanisms of Wolbachia-induced CI.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherAmerican Chemical Society
dc.relation.ispartofpagefrom3835
dc.relation.ispartofpageto3847
dc.relation.ispartofissue9
dc.relation.ispartofjournalJournal of Proteome Research
dc.relation.ispartofvolume14
dc.subject.fieldofresearchMicrobiology not elsewhere classified
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchChemical Sciences
dc.subject.fieldofresearchcode060599
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode03
dc.titleQuantitative proteomic analyses of molecular mechanisms associated with cytoplasmic incompatibility in drosophila melanogaster induced by wolbachia
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorBrownlie, Jeremy


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