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dc.contributor.authorBohlmann, Lisa
dc.contributor.authorTredwell, Gregory D
dc.contributor.authorYu, Xing
dc.contributor.authorChang, Chih-Wei
dc.contributor.authorHaselhorst, Thomas
dc.contributor.authorWinger, Moritz
dc.contributor.authorDyason, Jeffrey C
dc.contributor.authorThomson, Robin J
dc.contributor.authorTiralongo, Joe
dc.contributor.authorBeacham, Ifor R
dc.contributor.authorBlanchard, Helen
dc.contributor.authorvon Itzstein, Mark
dc.date.accessioned2017-11-27T05:09:00Z
dc.date.available2017-11-27T05:09:00Z
dc.date.issued2015
dc.identifier.issn1552-4450
dc.identifier.doi10.1038/nchembio.1956
dc.identifier.urihttp://hdl.handle.net/10072/101717
dc.description.abstractWe report the structural and functional characterization of a novel heparanase (BpHep) from the invasive pathogenic bacterium Burkholderia pseudomallei (Bp), showing ∼24% sequence identity with human heparanase (hHep). Site-directed mutagenesis studies confirmed the active site resi-dues essential for activity, and we found that BpHep has specificity for heparan sulfate. Finally, we describe the first heparanase X-ray crystal structure, which provides new insight into both substrate recognition and inhibitor design.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherNature Publishing Group
dc.relation.ispartofpagefrom955
dc.relation.ispartofpageto957
dc.relation.ispartofissue12
dc.relation.ispartofjournalNature Chemical Biology
dc.relation.ispartofvolume11
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry not elsewhere classified
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classified
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchcode030499
dc.subject.fieldofresearchcode060199
dc.subject.fieldofresearchcode0304
dc.subject.fieldofresearchcode0601
dc.titleFunctional and structural characterization of a heparanase
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyOffice of the Snr Dep Vice Chancellor, Institute for Glycomics
gro.hasfulltextNo Full Text
gro.griffith.authorBeacham, Ifor R.
gro.griffith.authorvon Itzstein, Mark
gro.griffith.authorThomson, Robin J.
gro.griffith.authorDyason, Jeffrey C.
gro.griffith.authorHaselhorst, Thomas E.
gro.griffith.authorTredwell, Greg
gro.griffith.authorBlanchard, Helen
gro.griffith.authorTiralongo, Joe
gro.griffith.authorYu, Xing
gro.griffith.authorBohlmann, Lisa
gro.griffith.authorWinger, Moritz
gro.griffith.authorChang, Chih-Wei


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