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  • The Haemonchus contortus kinome - A resource for fundamental molecular investigations and drug discovery

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    StroehleinPUB754.pdf (823.3Kb)
    Author(s)
    Stroehlein, Andreas J
    Young, Neil D
    Korhonen, Pasi K
    Jabbar, Abdul
    Hofmann, Andreas
    Sternberg, Paul W
    Gasser, Robin B
    Griffith University Author(s)
    Hofmann, Andreas
    Year published
    2015
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    Abstract
    Background: Protein kinases regulate a plethora of essential signalling and other biological pathways in all eukaryotic organisms, but very little is known about them in most parasitic nematodes. Methods: Here, we defined, for the first time, the entire complement of protein kinases (kinome) encoded in the barber’s pole worm (Haemonchus contortus) through an integrated analysis of transcriptomic and genomic datasets using an advanced bioinformatic workflow. Results: We identified, curated and classified 432 kinases representing ten groups, 103 distinct families and 98 subfamilies. A comparison of the kinomes of H. contortus ...
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    Background: Protein kinases regulate a plethora of essential signalling and other biological pathways in all eukaryotic organisms, but very little is known about them in most parasitic nematodes. Methods: Here, we defined, for the first time, the entire complement of protein kinases (kinome) encoded in the barber’s pole worm (Haemonchus contortus) through an integrated analysis of transcriptomic and genomic datasets using an advanced bioinformatic workflow. Results: We identified, curated and classified 432 kinases representing ten groups, 103 distinct families and 98 subfamilies. A comparison of the kinomes of H. contortus and Caenorhabditis elegans (a related, free-living nematode) revealed considerable variation in the numbers of casein kinases, tyrosine kinases and Ca2+/calmodulin-dependent protein kinases, which likely relate to differences in biology, habitat and life cycle between these worms. Moreover, a suite of kinase genes was selectively transcribed in particular developmental stages of H. contortus, indicating central roles in developmental and reproductive processes. In addition, using a ranking system, drug targets (n = 13) and associated small-molecule effectors (n = 1517) were inferred. Conclusions: The H. contortus kinome will provide a useful resource for fundamental investigations of kinases and signalling pathways in this nematode, and should assist future anthelmintic discovery efforts; this is particularly important, given current drug resistance problems in parasitic nematodes.
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    Journal Title
    Parasites and Vectors
    Volume
    8
    DOI
    https://doi.org/10.1186/s13071-015-1231-5
    Copyright Statement
    © 2015 Stroehlein et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
    Note
    Page numbers are not for citation purposes. Instead, this article has the unique article number of 623.
    Subject
    Medical Microbiology not elsewhere classified
    Medical Microbiology
    Public Health and Health Services
    Publication URI
    http://hdl.handle.net/10072/101726
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    • Journal articles

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