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dc.contributor.authorStroehlein, Andreas J
dc.contributor.authorYoung, Neil D
dc.contributor.authorKorhonen, Pasi K
dc.contributor.authorJabbar, Abdul
dc.contributor.authorHofmann, Andreas
dc.contributor.authorSternberg, Paul W
dc.contributor.authorGasser, Robin B
dc.date.accessioned2017-05-25T01:37:30Z
dc.date.available2017-05-25T01:37:30Z
dc.date.issued2015
dc.identifier.issn1756-3305
dc.identifier.doi10.1186/s13071-015-1231-5
dc.identifier.urihttp://hdl.handle.net/10072/101726
dc.description.abstractBackground: Protein kinases regulate a plethora of essential signalling and other biological pathways in all eukaryotic organisms, but very little is known about them in most parasitic nematodes. Methods: Here, we defined, for the first time, the entire complement of protein kinases (kinome) encoded in the barber’s pole worm (Haemonchus contortus) through an integrated analysis of transcriptomic and genomic datasets using an advanced bioinformatic workflow. Results: We identified, curated and classified 432 kinases representing ten groups, 103 distinct families and 98 subfamilies. A comparison of the kinomes of H. contortus and Caenorhabditis elegans (a related, free-living nematode) revealed considerable variation in the numbers of casein kinases, tyrosine kinases and Ca2+/calmodulin-dependent protein kinases, which likely relate to differences in biology, habitat and life cycle between these worms. Moreover, a suite of kinase genes was selectively transcribed in particular developmental stages of H. contortus, indicating central roles in developmental and reproductive processes. In addition, using a ranking system, drug targets (n = 13) and associated small-molecule effectors (n = 1517) were inferred. Conclusions: The H. contortus kinome will provide a useful resource for fundamental investigations of kinases and signalling pathways in this nematode, and should assist future anthelmintic discovery efforts; this is particularly important, given current drug resistance problems in parasitic nematodes.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherBioMed Central
dc.relation.ispartofpagefrom623-1
dc.relation.ispartofpageto623-13
dc.relation.ispartofjournalParasites and Vectors
dc.relation.ispartofvolume8
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchMedical microbiology not elsewhere classified
dc.subject.fieldofresearchcode3207
dc.subject.fieldofresearchcode320799
dc.titleThe Haemonchus contortus kinome - A resource for fundamental molecular investigations and drug discovery
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.description.notepublicPage numbers are not for citation purposes. Instead, this article has the unique article number of 623.
gro.rights.copyright© 2015 Stroehlein et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
gro.hasfulltextFull Text
gro.griffith.authorHofmann, Andreas


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