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dc.contributor.authorKakkanat, Asha
dc.contributor.authorTotsika, Makrina
dc.contributor.authorSchaale, Kolja
dc.contributor.authorDuell, Benjamin L
dc.contributor.authorLo, Alvin W
dc.contributor.authorPhan, Minh-Duy
dc.contributor.authorMoriel, Danilo G
dc.contributor.authorBeatson, Scott A
dc.contributor.authorSweet, Matthew J
dc.contributor.authorUlett, Glen C
dc.contributor.authorSchembri, Mark A
dc.date.accessioned2017-05-26T01:31:36Z
dc.date.available2017-05-26T01:31:36Z
dc.date.issued2015
dc.identifier.issn2045-2322
dc.identifier.doi10.1038/srep16149
dc.identifier.urihttp://hdl.handle.net/10072/101761
dc.description.abstractEscherichia coli sequence type 131 (ST131) is a globally dominant multidrug resistant clone associated with urinary tract and bloodstream infections. Most ST131 strains exhibit resistance to multiple antibiotics and cause infections associated with limited treatment options. The largest sub-clonal ST131 lineage is resistant to fluoroquinolones, contains the type 1 fimbriae fimH30 allele and expresses an H4 flagella antigen. Flagella are motility organelles that contribute to UPEC colonisation of the upper urinary tract. In this study, we examined the specific role of H4 flagella in ST131 motility and interaction with host epithelial and immune cells. We show that the majority of H4-positive ST131 strains are motile and are enriched for flagella expression during static pellicle growth. We also tested the role of H4 flagella in ST131 through the construction of specific mutants, over-expression strains and isogenic mutants that expressed alternative H1 and H7 flagellar subtypes. Overall, our results revealed that H4, H1 and H7 flagella possess conserved phenotypes with regards to motility, epithelial cell adhesion, invasion and uptake by macrophages. In contrast, H4 flagella trigger enhanced induction of the anti-inflammatory cytokine IL-10 compared to H1 and H7 flagella, a property that may contribute to ST131 fitness in the urinary tract.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Macmillan
dc.relation.ispartofpagefrom16149-1
dc.relation.ispartofpageto16149-14
dc.relation.ispartofjournalScientific Reports
dc.relation.ispartofvolume5
dc.relation.urihttp://purl.org/au-research/grants/NHMRC/APP1084889
dc.relation.grantIDAPP1084889
dc.relation.fundersNHMRC
dc.subject.fieldofresearchBiochemistry and cell biology not elsewhere classified
dc.subject.fieldofresearchcode310199
dc.titleThe role of H4 flagella in Escherichia coli ST131 virulence
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2015. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
gro.hasfulltextFull Text
gro.griffith.authorUlett, Glen C.


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