dc.contributor.author | Chakravorty, Nishant | |
dc.contributor.author | Hamlet, Stephen | |
dc.contributor.author | Jaiprakash, Anjali | |
dc.contributor.author | Crawford, Ross | |
dc.contributor.author | Oloyede, Adekunle | |
dc.contributor.author | Alfarsi, Mohammed | |
dc.contributor.author | Xiao, Yin | |
dc.contributor.author | Ivanovski, Saso | |
dc.date.accessioned | 2017-10-05T02:27:42Z | |
dc.date.available | 2017-10-05T02:27:42Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0905-7161 | |
dc.identifier.doi | 10.1111/clr.12178 | |
dc.identifier.uri | http://hdl.handle.net/10072/102409 | |
dc.description.abstract | Objectives:
Titanium implant surfaces with modified topographies have improved osteogenic properties in vivo. However, the molecular mechanisms remain obscure. This study explored the signaling pathways responsible for the pro-osteogenic properties of micro-roughened (SLA) and chemically/nanostructurally (modSLA) modified titanium surfaces on human alveolar bone-derived osteoprogenitor cells (BCs) in vitro.
Materials and methods:
The activation of stem cell signaling pathways (TGFβ/BMP, Wnt, FGF, Hedgehog, Notch) was investigated following early exposure (24 and 72 h) of BCs to SLA and modSLA surfaces in the absence of osteogenic cell culture supplements.
Results:
Key regulatory genes from the TGFβ/BMP (TGFBR2, BMPR2, BMPR1B, ACVR1B, SMAD1, SMAD5), Wnt (Wnt/β-catenin and Wnt/Ca2+) (FZD1, FZD3, FZD5, LRP5, NFATC1, NFATC2, NFATC4, PYGO2, LEF1) and Notch (NOTCH1, NOTCH2, NOTCH4, PSEN1, PSEN2, PSENEN) pathways were upregulated on the modified surfaces. These findings correlated with a higher expression of osteogenic markers bone sialoprotein (IBSP) and osteocalcin (BGLAP), and bone differentiation factors BMP2, BMP6, and GDF15, as observed on the modified surfaces.
Conclusions:
These findings demonstrate that the activation of the pro-osteogenic cell signaling pathways by modSLA and SLA surfaces leads to enhanced osteogenic differentiation as evidenced after 7 and 14 days culture in osteogenic media and provides a mechanistic insight into the superior osseointegration on the modified surfaces observed in vivo. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Wiley-Blackwell Publishing | |
dc.publisher.place | United States | |
dc.relation.ispartofpagefrom | 475 | |
dc.relation.ispartofpageto | 486 | |
dc.relation.ispartofissue | 4 | |
dc.relation.ispartofjournal | Clinical Oral Implants Research | |
dc.relation.ispartofvolume | 25 | |
dc.relation.uri | http://purl.org/au-research/grants/NHMRC/APP1036625 | |
dc.relation.grantID | APP1036625 | |
dc.relation.funders | NHMRC | |
dc.subject.fieldofresearch | Biomedical engineering | |
dc.subject.fieldofresearch | Biomedical engineering not elsewhere classified | |
dc.subject.fieldofresearch | Dentistry | |
dc.subject.fieldofresearch | Dentistry not elsewhere classified | |
dc.subject.fieldofresearch | Clinical sciences | |
dc.subject.fieldofresearchcode | 4003 | |
dc.subject.fieldofresearchcode | 400399 | |
dc.subject.fieldofresearchcode | 3203 | |
dc.subject.fieldofresearchcode | 320399 | |
dc.subject.fieldofresearchcode | 3202 | |
dc.title | Pro-osteogenic topographical cues promote early activation of osteoprogenitor differentiation via enhanced TGFβ, Wnt, and Notch signaling | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Xiao, Yin | |