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dc.contributor.authorChakravorty, Nishant
dc.contributor.authorHamlet, Stephen
dc.contributor.authorJaiprakash, Anjali
dc.contributor.authorCrawford, Ross
dc.contributor.authorOloyede, Adekunle
dc.contributor.authorAlfarsi, Mohammed
dc.contributor.authorXiao, Yin
dc.contributor.authorIvanovski, Saso
dc.date.accessioned2017-10-05T02:27:42Z
dc.date.available2017-10-05T02:27:42Z
dc.date.issued2014
dc.identifier.issn0905-7161
dc.identifier.doi10.1111/clr.12178
dc.identifier.urihttp://hdl.handle.net/10072/102409
dc.description.abstractObjectives: Titanium implant surfaces with modified topographies have improved osteogenic properties in vivo. However, the molecular mechanisms remain obscure. This study explored the signaling pathways responsible for the pro-osteogenic properties of micro-roughened (SLA) and chemically/nanostructurally (modSLA) modified titanium surfaces on human alveolar bone-derived osteoprogenitor cells (BCs) in vitro. Materials and methods: The activation of stem cell signaling pathways (TGFβ/BMP, Wnt, FGF, Hedgehog, Notch) was investigated following early exposure (24 and 72 h) of BCs to SLA and modSLA surfaces in the absence of osteogenic cell culture supplements. Results: Key regulatory genes from the TGFβ/BMP (TGFBR2, BMPR2, BMPR1B, ACVR1B, SMAD1, SMAD5), Wnt (Wnt/β-catenin and Wnt/Ca2+) (FZD1, FZD3, FZD5, LRP5, NFATC1, NFATC2, NFATC4, PYGO2, LEF1) and Notch (NOTCH1, NOTCH2, NOTCH4, PSEN1, PSEN2, PSENEN) pathways were upregulated on the modified surfaces. These findings correlated with a higher expression of osteogenic markers bone sialoprotein (IBSP) and osteocalcin (BGLAP), and bone differentiation factors BMP2, BMP6, and GDF15, as observed on the modified surfaces. Conclusions: These findings demonstrate that the activation of the pro-osteogenic cell signaling pathways by modSLA and SLA surfaces leads to enhanced osteogenic differentiation as evidenced after 7 and 14 days culture in osteogenic media and provides a mechanistic insight into the superior osseointegration on the modified surfaces observed in vivo.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing
dc.publisher.placeUnited States
dc.relation.ispartofpagefrom475
dc.relation.ispartofpageto486
dc.relation.ispartofissue4
dc.relation.ispartofjournalClinical Oral Implants Research
dc.relation.ispartofvolume25
dc.relation.urihttp://purl.org/au-research/grants/NHMRC/APP1036625
dc.relation.grantIDAPP1036625
dc.relation.fundersNHMRC
dc.subject.fieldofresearchBiomedical engineering
dc.subject.fieldofresearchBiomedical engineering not elsewhere classified
dc.subject.fieldofresearchDentistry
dc.subject.fieldofresearchDentistry not elsewhere classified
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchcode4003
dc.subject.fieldofresearchcode400399
dc.subject.fieldofresearchcode3203
dc.subject.fieldofresearchcode320399
dc.subject.fieldofresearchcode3202
dc.titlePro-osteogenic topographical cues promote early activation of osteoprogenitor differentiation via enhanced TGFβ, Wnt, and Notch signaling
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorXiao, Yin


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