Enterocyte and M-Cell Transport of Native and Heat-Denatured Bovine β-Lactoglobulin: Significance of Heat Denaturation
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The three-dimensional structure, digestibility, and immunological properties of bovine ߭lactoglobulin (߭lg) are modified by heat treatments used in processing of liquid milk products. Because it is not known if such treatments also modify the intestinal transport properties of ߭lg, the transport of native and heat-denatured bovine ߭lg was investigated in experimental cell models using Caco-2 cells and M cells. Transport of ߭lg labeled with a fluorescent marker was followed with fluorometric measurements, electrophoretic analyses, and fluorescence microscopy. The data show that both cell types transported native ߭lg more efficiently than they did heat-denatured ߭lg. In addition, M cells transported native ߭lg more than Caco-2 cells. Transport of native and heat-denatured ߭lg was transcellular. The electrophoretic data also suggest that heat-denatured ߭lg may have degraded more than native ߭lg during the transport
Journal of Agricriculture and Food Chemistry
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