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dc.contributor.authorAgranovski, Igoren_US
dc.contributor.authorSafatov, A.en_US
dc.contributor.authorSergeev, A.en_US
dc.contributor.authorPyankov, Olegen_US
dc.contributor.authorPetrishchenko, V.en_US
dc.contributor.authorMikheev, M.en_US
dc.contributor.authorSergeev, A.en_US
dc.contributor.editorProf Brimblecombeen_US
dc.date.accessioned2017-04-24T10:13:36Z
dc.date.available2017-04-24T10:13:36Z
dc.date.issued2006en_US
dc.date.modified2009-10-09T06:10:53Z
dc.identifier.issn13522310en_US
dc.identifier.doi10.1016/j.atmosenv.2006.02.023en_AU
dc.identifier.urihttp://hdl.handle.net/10072/11465
dc.description.abstractA new personal sampler had been previously developed and verified for monitoring of viable airborne viruses. The aims of this project were to investigate a possibility of the utilization of the polymerase chain reaction (PCR) method to speed up the time consuming analytical procedures and to evaluate a lower detection limit of the combined (sampler-PCR) device. Tenfold serial dilutions of the initial suspension of the Vaccinia virus were aerosolized in the chamber and airborne viruses were monitored by two simultaneously operating samplers. The results of monitoring were successfully obtained by a standard plaque assay (live microbes) and by the PCR method (total DNA). The corresponding calculations to identify the minimal detectable concentration in the ambient air were then performed. It was found that the minimal detectable concentration of airborne viruses in the ambient air depends on the sampling time. As demonstrated, such concentration should be at least 125ױ03 PFU m-3 for a sampling time of as short as 1 min. The detectable concentration decreases with the increase of the sampling time and reaches 25ױ03 and 10ױ03 PFU m-3 for 5 and 12.5 min of sampling respectively.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherTaylor & Francis Inc.en_US
dc.publisher.placeOxford, UKen_US
dc.publisher.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/246/description#descriptionen_AU
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom3924en_US
dc.relation.ispartofpageto3929en_US
dc.relation.ispartofjournalAtmospheric Environmenten_US
dc.relation.ispartofvolume40en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchcode270304en_US
dc.titleRapid detection of airborne viruses by personal bioaerosol sampler combined with the PCR device.en_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyGriffith Sciences, Griffith School of Engineeringen_US
gro.date.issued2006
gro.hasfulltextNo Full Text


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