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dc.contributor.authorCheung, B
dc.contributor.authorHolmes, RS
dc.contributor.authorEasteal, S
dc.contributor.authorBeacham, IR
dc.date.accessioned2020-02-11T05:55:41Z
dc.date.available2020-02-11T05:55:41Z
dc.date.issued1999
dc.identifier.issn0737-4038
dc.identifier.doi10.1093/oxfordjournals.molbev.a026035
dc.identifier.urihttp://hdl.handle.net/10072/118653
dc.description.abstractAbstract The three class I alcohol dehydrogenases (ADHs) in humans comprise homo- and heterodimers of three subunits (alpha, beta, and gamma) with greater than 90% sequence identity. These are encoded by distinct genes (ADH1, ADH2, and ADH3, respectively) and are all expressed in the liver. In baboons, only the beta ADH subunit is expressed in liver. A second class I ADH is expressed in the kidney; we isolated, cloned, and sequenced the cDNA corresponding to this ADH and conclude that it is of the gamma ADH lineage. We also amplified and sequenced the 5' noncoding regions of all three class I baboon ADH genes and the rhesus monkey ADH1 gene and compared their nucleotide sequences with the corresponding human sequences. There is clear evidence that the evolution of these genes has been reticulate. At least three gene conversion events, affecting the coding and 3' noncoding regions of the genes, are inferred from compatibility and partition matrices and phylogenetic analysis of the sequences. Our estimation of the evolutionary history of these genes provides a framework for the investigation of relative substitution rates and functional variation among the sequences. Relative-rate tests, designed to account for the reticulate evolution of these genes, indicate no difference in substitution rate either between genes encoding different subunits or between human and Old World monkey lineages. The human and baboon gamma ADH sequences do not show clear differences at functionally important sites within the coding region, but they do differ at a number of sites in regions previously proposed to be regulatory sites for transcriptional control. This variation may explain the different patterns of gene expression in humans and baboons.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherOxford University Press
dc.publisher.placeUK, Australia
dc.relation.ispartofpagefrom23
dc.relation.ispartofpageto36
dc.relation.ispartofissue1
dc.relation.ispartofjournalMolecular Biology and Evolution
dc.relation.ispartofvolume16
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchEvolutionary biology
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3104
dc.subject.fieldofresearchcode3105
dc.titleEvolution of Class I Alcohol Dehydrogenase Genes in Catarrhine Primates: Gene Conversion, Substitution Rates, and Gene Regulation
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyAn Unassigned Group, An Unassigned Department
gro.hasfulltextNo Full Text
gro.griffith.authorHolmes, Roger S.
gro.griffith.authorBeacham, Ifor R.


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