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  • A rapid, direct measurement of bacterial growth rate in anaerobic wastewater treatment

    Author
    Pollard, Peter
    Year published
    2006
    Metadata
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    Abstract
    Reliable design and operation of biological wastewater treatment systems demand robust models of biological degradation processes. However, methods to directly measure key bacterial growth kinetics have not been readily available. Those methods that are available rely on the classic measurement of aerobic respiration using oxygen uptake take rates. This paper shows how the thymidine assay can be used as a rapid and direct measurement of bacterial specific growth rates (婠in situ for an anaerobic treatment process, independent of aerobic respiration. A filtration-based assay is applied and evaluated a dispersed-phase high-rate ...
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    Reliable design and operation of biological wastewater treatment systems demand robust models of biological degradation processes. However, methods to directly measure key bacterial growth kinetics have not been readily available. Those methods that are available rely on the classic measurement of aerobic respiration using oxygen uptake take rates. This paper shows how the thymidine assay can be used as a rapid and direct measurement of bacterial specific growth rates (婠in situ for an anaerobic treatment process, independent of aerobic respiration. A filtration-based assay is applied and evaluated a dispersed-phase high-rate anaerobic treatment process, with results obtained in less than an hour. The chemical oxygen demand (COD) biomass in the reactor was 0.52 kg COD m -3 and the specific growth rate of these anaerobic bacteria was 0.8 ᠰ.2 d -1. It took the bacterial populations 21.6 hours to double. This is an important advancement from existing methods that use aerobic respiration as a pseudo measurement of bacterial specific growth rates. The method allows rapid and direct measures of microbial growth rates for anaerobic treatment processes.
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    Journal Title
    Water Environment Research
    Volume
    78
    Issue
    2
    Publisher URI
    http://www.ingentaconnect.com/content/wef/wer
    DOI
    https://doi.org/10.2175/106143005X89643
    Publication URI
    http://hdl.handle.net/10072/11928
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    • Journal articles

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