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dc.contributor.authorLewohl, JM
dc.contributor.authorCrane, DI
dc.contributor.authorDodd, PR
dc.date.accessioned2019-05-27T01:36:15Z
dc.date.available2019-05-27T01:36:15Z
dc.date.issued1997
dc.identifier.issn1385-299X
dc.identifier.doi10.1016/S1385-299X(97)00009-3
dc.identifier.urihttp://hdl.handle.net/10072/121496
dc.description.abstractThe GABAA receptor is the site of action of the inhibitory neurotransmitter GABA, as well as a number of pharmacologically important drugs such as benzodiazepines, barbiturates, and ethanol. The GABAA receptor is a pentameric complex composed of distinct polypeptides, which have been divided into five subunit classes on the basis of sequence homology. To date, 17 isoforms of the receptor have been identified and cloned in mammalian brain, and designated α1–6, β1–4, γ1–4, δ and ρ1–2. In addition, several isoforms exist in alternatively spliced forms (for review see ref. [13]). Studies on recombinant receptors have revealed that receptors constituted from different isoforms exhibit distinct pharmacological properties. For example, the α subunit class appears to be responsible for GABA enhancement of benzodiazepine binding [15]. GABAA receptor function is modulated by benzodiazepine agonists such as flunitrazepam and diazepam, barbiturates, anaesthetics, neurosteroids, and ethanol. Chronic treatment of animals with many of these compounds can bring about profound changes in receptor expression and pharmacology [12]. The RT/PCR assay described here was developed to quantify the α1, α2 and α3 isoforms in the same assay. The amount of each isoform was quantified on the basis of a standard curve generated under identical PCR conditions to the target sequences. In this way it is possible to quantify multiple samples in each RT/PCR assay, thereby reducing inter-assay variability. The assay can be applied to quantify the expression of these isoforms in response to acute and chronic drug administration, or in particular disease states. Altered expression may reflect a corresponding change in protein synthesis, or an alteration of the subtype composition of GABAA receptor.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier Science
dc.publisher.placeNetherlands
dc.relation.ispartofpagefrom347
dc.relation.ispartofpageto356
dc.relation.ispartofissue4
dc.relation.ispartofjournalBrain Research Protocols
dc.relation.ispartofvolume1
dc.subject.fieldofresearchNeurosciences
dc.subject.fieldofresearchCognitive and computational psychology
dc.subject.fieldofresearchBiological psychology
dc.subject.fieldofresearchcode3209
dc.subject.fieldofresearchcode5204
dc.subject.fieldofresearchcode5202
dc.titleA method for the quantitation of the α1, α2 and α3 isoforms of the GABAA receptor in human brain using competitive PCR
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medical Science
gro.hasfulltextNo Full Text
gro.griffith.authorCrane, Denis I.
gro.griffith.authorLewohl, Joanne M.


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