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dc.contributor.authorMagot, Michel
dc.contributor.authorFardeau, Marie-Laure
dc.contributor.authorArnauld, Odile
dc.contributor.authorLanau, Colette
dc.contributor.authorOllivier, Bernard
dc.contributor.authorThomas, P.
dc.contributor.authorPatel, Bharat
dc.date.accessioned2019-05-27T00:02:18Z
dc.date.available2019-05-27T00:02:18Z
dc.date.issued1997
dc.identifier.issn03781097
dc.identifier.doi10.1111/j.1574-6968.1997.tb13876.x
dc.identifier.urihttp://hdl.handle.net/10072/121509
dc.description.abstractAn obligately anaerobic spirochete designated strain SEBR 4228T (T = type strain) was isolated from an oil field of Congo, Central Africa. The strain grew optimally with a sodium chloride concentration of 5% (sodium chloride concentration growth range 1.0–10%) at 37°C (growth temperature range 20–40°C) and pH of 7.0–7.2 (pH growth range pH 5.5–8.0). Strain SEBR 4228T grew on carbohydrates (glucose, fructose, ribose, D-xylose, galactose, mannitol and mannose), glycerol, fumarate, peptides and yeast extract. Yeast extract was required for growth and could not be replaced by vitamins. It reduced thiosulfate and sulfur, to H2S. Glucose was oxidised to lactate, acetate, CO2 and H2S in the presence of thiosulfate but in its absence lactate, ethanol, CO2 and H2 were produced. Fumarate was fermented to acetate and succinate. The G+C content of strain SEBR 4228T was 50%. Strain SEBR 4228T was spiral shaped measuring 5–30 by 0.3–0.5 μm and was motile with a corkscrew-like motion. Electron microscopy revealed the presence of periplasmic flagella in a 1-2-1 arrangement. Strain SEBR 4228T possessed features typical of the members of the genus Spirochaeta. 16S rRNA sequence analysis revealed that it was closely related to Spirochaeta bajacaliforniensis (similarity 98.6%). The lack of DNA homology with S. bajacaliforniensis (38%), together with other phenotypic differences, indicated that strain SEBR 4228T is a new species, which we have designated Spirochaeta smaragdinae. The type strain is SEBR 4228T (= DSM 11293).
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier Science
dc.publisher.placeNetherlands, UK
dc.relation.ispartofpagefrom185
dc.relation.ispartofpageto191
dc.relation.ispartofissue2
dc.relation.ispartofjournalFEMS Microbiology Letters
dc.relation.ispartofvolume155
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchAgricultural and Veterinary Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode07
dc.subject.fieldofresearchcode11
dc.titleSpirochaeta Smaragdinae sp. nov., a New Mesophilic Strictly Anaerobic Spirochete from an Oil Field
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorPatel, Bharat K.


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