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dc.contributor.authorWoo, TH
dc.contributor.authorPatel, BK
dc.contributor.authorSmythe, LD
dc.contributor.authorSymonds, ML
dc.contributor.authorNorris, MA
dc.contributor.authorDohnt, MF
dc.date.accessioned2019-05-27T00:18:08Z
dc.date.available2019-05-27T00:18:08Z
dc.date.issued1997
dc.identifier.issn0378-1097
dc.identifier.doi10.1111/j.1574-6968.1997.tb13874.x
dc.identifier.urihttp://hdl.handle.net/10072/121520
dc.description.abstractBased on (i) an analysis of Leptospira 16S rDNA sequences determined by us and of those from databases and (ii) a previously published finding that restriction fragment length polymorphisms (RFLPs) within the Leptospira 16S and 23S rDNA were detected by nine restriction enzymes and these RFLPs allowed categorisation of Leptospira into eight genospecies, we predicted that one particular DdeI restriction site polymorphism within 16S rDNA could be independently used for identifications of Leptospira strains belonging to the genospecies interrogans. Two PCR-based methods, namely allele-specific amplification (ASA) and PCR-RFLP, were tested for the rapid detection of the DdeI restriction site polymorphism. One or two representative strains from each of nine genospecies were tested by ASA, whereas 73 strains from nine genospecies and two field isolates were tested by PCR-RFLP. Our experiments showed that the ASA method was not as specific as intended, but the PCR-RFLP method was useful for rapid identifications of the genospecies interrogans. We have not only confirmed a previous finding and extended the number of samples particularly from the genospecies biflexa, weilii, and inadai, but also simplified a previous PCR-RFLP protocol.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier Science
dc.publisher.placeNetherlands, UK
dc.relation.ispartofpagefrom169
dc.relation.ispartofpageto177
dc.relation.ispartofissue2
dc.relation.ispartofjournalFEMS Microbiology Letters
dc.relation.ispartofvolume155
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchAgricultural, veterinary and food sciences
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode30
dc.subject.fieldofresearchcode32
dc.titleComparison of two PCR Methods for Rapid Identification of Leptospira Genospecies Interrogans
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Public Health
gro.hasfulltextNo Full Text
gro.griffith.authorPatel, Bharat K.
gro.griffith.authorWoo, Hok


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