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  • Discovery of Molecular Markers to Discriminate Corneal Endothelial Cells in the Human Body

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    Version of Record (VoR)
    Author(s)
    Yoshihara, Masahito
    Ohmiya, Hiroko
    Hara, Susumu
    Kawasaki, Satoshi
    Hayashizaki, Yoshihide
    Itoh, Masayoshi
    Kawaji, Hideya
    Tsujikawa, Motokazu
    Nishida, Kohji
    Griffith University Author(s)
    Mackay-Sim, Alan
    Year published
    2015
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    Abstract
    he corneal endothelium is a monolayer of hexagonal corneal endothelial cells (CECs) on the inner surface of the cornea. CECs are critical in maintaining corneal transparency through their barrier and pump functions. CECs in vivo have a limited capacity in proliferation, and loss of a significant number of CECs results in corneal edema called bullous keratopathy which can lead to severe visual loss. Corneal transplantation is the most effective method to treat corneal endothelial dysfunction, where it suffers from donor shortage. Therefore, regeneration of CECs from other cell types attracts increasing interests, and specific ...
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    he corneal endothelium is a monolayer of hexagonal corneal endothelial cells (CECs) on the inner surface of the cornea. CECs are critical in maintaining corneal transparency through their barrier and pump functions. CECs in vivo have a limited capacity in proliferation, and loss of a significant number of CECs results in corneal edema called bullous keratopathy which can lead to severe visual loss. Corneal transplantation is the most effective method to treat corneal endothelial dysfunction, where it suffers from donor shortage. Therefore, regeneration of CECs from other cell types attracts increasing interests, and specific markers of CECs are crucial to identify actual CECs. However, the currently used markers are far from satisfactory because of their non-specific expression in other cell types. Here, we explored molecular markers to discriminate CECs from other cell types in the human body by integrating the published RNA-seq data of CECs and the FANTOM5 atlas representing diverse range of cell types based on expression patterns. We identified five genes, CLRN1, MRGPRX3, HTR1D, GRIP1 and ZP4 as novel markers of CECs, and the specificities of these genes were successfully confirmed by independent experiments at both the RNA and protein levels. Notably none of them have been documented in the context of CEC function. These markers could be useful for the purification of actual CECs, and also available for the evaluation of the products derived from other cell types. Our results demonstrate an effective approach to identify molecular markers for CECs and open the door for the regeneration of CECs in vitro.
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    Journal Title
    PLoS One
    Volume
    10
    Issue
    5
    DOI
    https://doi.org/10.1371/journal.pone.0117581
    Copyright Statement
    © 2015 Yoshihara et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
    Subject
    Medical Genetics (excl. Cancer Genetics)
    Publication URI
    http://hdl.handle.net/10072/123422
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    • Journal articles

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