Photocatalytic inactivation of Escherichia coli—The roles of genes in β-oxidation of fatty acid degradation
Author(s)
Chu, Ka Him
Huang, Guocheng
An, Taicheng
Li, Guiying
Yip, Pui Ling
Ng, Tsz Wai
Yip, Ho Yin
Zhao, Huijun
Wong, Po Keung
Griffith University Author(s)
Year published
2016
Metadata
Show full item recordAbstract
A genetic study on the relationship between cell membrane fatty acid profile and photocatalytic inactivation efficiency of Escherichia coli was conducted. Photocatalytic inactivation of a parental strain (E. coli BW25113) and its seven isogenic mutants with deletion of single gene involving in β-oxidation of fatty acid degradation (fad) were compared. Most of the mutants involved in fatty acid degradation did not show significant difference in susceptibility towards photocatalytic inactivation compared with the parental strain, except that E. coli JW1176 (fadR mutant) and E. coli JW3935 (fabR mutant) showed a lower and higher ...
View more >A genetic study on the relationship between cell membrane fatty acid profile and photocatalytic inactivation efficiency of Escherichia coli was conducted. Photocatalytic inactivation of a parental strain (E. coli BW25113) and its seven isogenic mutants with deletion of single gene involving in β-oxidation of fatty acid degradation (fad) were compared. Most of the mutants involved in fatty acid degradation did not show significant difference in susceptibility towards photocatalytic inactivation compared with the parental strain, except that E. coli JW1176 (fadR mutant) and E. coli JW3935 (fabR mutant) showed a lower and higher sensitivity than the parental strain, respectively. Fluorescence microscopic analysis showed that the loss of cell permeability preceded the inactivation of bacterial cells. The results of temperature pre-treatment and fatty acid profiles of the parental strain and fadR mutant indicated that the alteration in cell membrane fatty acid composition played an important role, but not the most crucial one to affect the susceptibility of bacterial cell towards photocatalytic inactivation. The results in this study demonstrated the importance of cell membrane in the bacterial defense system against photocatalytic inactivation.
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View more >A genetic study on the relationship between cell membrane fatty acid profile and photocatalytic inactivation efficiency of Escherichia coli was conducted. Photocatalytic inactivation of a parental strain (E. coli BW25113) and its seven isogenic mutants with deletion of single gene involving in β-oxidation of fatty acid degradation (fad) were compared. Most of the mutants involved in fatty acid degradation did not show significant difference in susceptibility towards photocatalytic inactivation compared with the parental strain, except that E. coli JW1176 (fadR mutant) and E. coli JW3935 (fabR mutant) showed a lower and higher sensitivity than the parental strain, respectively. Fluorescence microscopic analysis showed that the loss of cell permeability preceded the inactivation of bacterial cells. The results of temperature pre-treatment and fatty acid profiles of the parental strain and fadR mutant indicated that the alteration in cell membrane fatty acid composition played an important role, but not the most crucial one to affect the susceptibility of bacterial cell towards photocatalytic inactivation. The results in this study demonstrated the importance of cell membrane in the bacterial defense system against photocatalytic inactivation.
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Journal Title
Catalysis Today
Volume
266
Subject
Chemical sciences
Genetic immunology
Engineering