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dc.contributor.authorPun, Ivan Ho Yuen
dc.contributor.authorChan, Dessy
dc.contributor.authorChan, Sau Hing
dc.contributor.authorChung, Po Yee
dc.contributor.authorZhou, Yuan Yuan
dc.contributor.authorLaw, Simon
dc.contributor.authorLam, Alfred King Yin
dc.contributor.authorChui, Chung Hin
dc.contributor.authorChan, Albert Sun Chi
dc.contributor.authorLam, Kim Hung
dc.contributor.authorTang, Johnny Cheuk On
dc.date.accessioned2017-08-27T23:07:28Z
dc.date.available2017-08-27T23:07:28Z
dc.date.issued2017
dc.identifier.issn1598-2998
dc.identifier.doi10.4143/crt.2016.190
dc.identifier.urihttp://hdl.handle.net/10072/124045
dc.description.abstractPurpose: 83b1 is a novel quinoline derivative that has been shown to inhibit cancer growth in human esophageal squamous cell carcinoma (ESCC). This study was conducted to comprehensively evaluate the cytotoxic effects of 83b1 on a series of ESCC cell lines and investigate the mechanisms by which 83b1 suppresses cancer growth based on molecular docking analysis. Materials and Methods: A series of ESCC and nontumor immortalized cell lines were exposed to 83b1 and cisplatin (CDDP) in a dose-dependent manner, and the cytotoxicity was examined by a MTS assay kit. Prediction of the molecular targets of 83b1 was conducted by molecular docking analysis. Expression of cyclooxygenase 2 (COX-2) mRNA and COX-2–derived prostaglandin E2 (PGE2) were measured by quantitative real-time polymerase chain reaction and enzymelinked immuno-sorbent assay, respectively. In vivo anti-tumor effect was determined using a nude mice xenografted model transplanted with an ESCC cell line, KYSE-450. Results: 83b1 showed the significant anti-cancer effects on all ESCC cell lines compared to CDDP; however, 83b1 revealed much lower toxic effects on non-tumor cell lines than CDDP. The predicted molecular target of 83b1 is peroxisome proliferator-activated receptor delta (PPARδ), which is a widely known oncoprotein. Additionally the expression of COX-2 mRNA and COX-2–derived PGE2 were down-regulated by 83b1 in a dose-dependent manner in ESCC cell lines. Furthermore, 83b1 was shown to significantly reduce the tumor size in nude mice xenograft. Conclusion: The results of this study suggest that the potential anti-cancer effects of 83b1 on human esophageal cancers occur through the possible oncotarget, PPARδ, and down-regulation of the cancer related genes and molecules.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherKorean Cancer Association
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto11
dc.relation.ispartofjournalCancer Research and Treatment
dc.subject.fieldofresearchCancer therapy (excl. chemotherapy and radiation therapy)
dc.subject.fieldofresearchcode321104
dc.titleAnti-Cancer Effects of a Novel Quinoline Derivative 83b1 on Human Esophageal Squamous Cell Carcinoma (ESCC) through Down-Regulation of COX-2 mRNA and PGE2
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by-nc/3.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2017 by the Korean Cancer Association. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorLam, Alfred K.


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