Comparison of test specificities of commercial antigen-based assays and in-house PCR methods for detection of rotavirus in stool specimens

Ye, S; Lambert, SB; Grimwood, K; Roczo-Farkas, S; Nimmo, GR; Sloots, TP; Kirkwood, CD; Whiley, DM

doi:10.1128/JCM.02251-14

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Author(s)

Ye, S

Lambert, SB

Grimwood, K

Roczo-Farkas, S

Nimmo, GR

Sloots, TP

Kirkwood, CD

Whiley, DM

Griffith University Author(s)

Nimmo, Graeme R.

Grimwood, Keith

Lambert, Stephen B.

Year published

2015

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Abstract

Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection.Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection.
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Journal Title

Journal of Clinical Microbiology

Volume

Issue

DOI

https://doi.org/10.1128/JCM.02251-14

Copyright Statement

© 2015 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.

Subject

Biological Sciences not elsewhere classified

Biological Sciences

Agricultural and Veterinary Sciences

Medical and Health Sciences

Publication URI

http://hdl.handle.net/10072/124995

Collection

Journal articles