Comparison of test specificities of commercial antigen-based assays and in-house PCR methods for detection of rotavirus in stool specimens

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Author(s)
Ye, S
Lambert, SB
Grimwood, K
Roczo-Farkas, S
Nimmo, GR
Sloots, TP
Kirkwood, CD
Whiley, DM
Griffith University Author(s)
Year published
2015
Metadata
Show full item recordAbstract
Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection.Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection.
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Journal Title
Journal of Clinical Microbiology
Volume
53
Issue
1
Copyright Statement
© 2015 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
Subject
Biological sciences
Other biological sciences not elsewhere classified
Agricultural, veterinary and food sciences
Biomedical and clinical sciences