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  • Comparison of test specificities of commercial antigen-based assays and in-house PCR methods for detection of rotavirus in stool specimens

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    Author(s)
    Ye, S
    Lambert, SB
    Grimwood, K
    Roczo-Farkas, S
    Nimmo, GR
    Sloots, TP
    Kirkwood, CD
    Whiley, DM
    Griffith University Author(s)
    Grimwood, Keith
    Year published
    2015
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    Abstract
    Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection.Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection.
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    Journal Title
    Journal of Clinical Microbiology
    Volume
    53
    Issue
    1
    DOI
    https://doi.org/10.1128/JCM.02251-14
    Copyright Statement
    © 2015 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
    Subject
    Biological sciences
    Other biological sciences not elsewhere classified
    Agricultural, veterinary and food sciences
    Biomedical and clinical sciences
    Publication URI
    http://hdl.handle.net/10072/124995
    Collection
    • Journal articles

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