A single Parathyroid Hormone (PTH) injection can accelerate the healing of stress fractures in rat ulnae
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Aim: Parathyroid hormone (PTH) has an anabolic effect that can accelerate bone remodelling. Therefore, our aim was to investigate the short-term effect of a single PTH injection on healing of SFx. Methods: Fifty-two female Wistar rats (300 g) were allocated to PTH and vehicle (VEH) groups. 24 hours after SFx loading. SFx was created in right ulnae of both groups (Figs 1 & 2). Groups were sub-divided into three groups, based on the time ulnae were harvested. Ulnae were harvested two, six and ten weeks after loading. Ulnae were dissected, processed for histology, stained with Toluidine blue and TRAP for osteoclasts count (Figs 3 & 4). Histomorphometry was conducted using OsteomeasureTM . Results: Measurements of cortical area, woven bone area or length of fracture did not differ significantly between the groups. Evidence of trends increasing SFx porosity (resorption), erosion andhealing area was observed in PTH groups, with a significant increase in osteoclast number after two weeks (P<0.01). Six weeks post SFx loading, Basic Multicellular Unit (BMU) size, healed bone area, healed bone perimeter, percentage healing, woven bone and healed bone areas were larger in PTH group. Woven bone apposition rate was significantly higher two weeks post loading in PTH group (P<0.01). The increased rate of bone healing continued to rise in PTH group, ten weeks post SFx. Conclusion: These data suggest that a single PTH injection, 24 hours after SFx initiation, results in active changes in bone remodelling dynamics to accelerate resorption after two weeks and healing after six weeks.
Australian New Zealand Bone & Mineral Society Annual Scientific Meeting 2015 in conjunction with Molecular & Experimental Pathology Society of Australasia (MEPSA) and Matrix Biology Society of Australia and New Zealand (MBSANZ)
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