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dc.contributor.authorHua, Yuanyuan
dc.contributor.authorChoi, Pui-Wah
dc.contributor.authorTrachtenberg, Alexander J
dc.contributor.authorNg, Allen C
dc.contributor.authorKuo, Winston P
dc.contributor.authorNg, Shu-Kay
dc.contributor.authorDinulescu, Daniela M
dc.contributor.authorMatzuk, Martin M
dc.contributor.authorBerkowitz, Ross S
dc.contributor.authorNg, Shu-Wing
dc.date.accessioned2018-02-20T00:59:28Z
dc.date.available2018-02-20T00:59:28Z
dc.date.issued2016
dc.identifier.issn1949-2553
dc.identifier.doi10.18632/oncotarget.11808
dc.identifier.urihttp://hdl.handle.net/10072/134142
dc.description.abstractEpithelial ovarian carcinoma accounts for 90% of all ovarian cancer and is the most deadly gynecologic malignancy. Recent studies have suggested that fallopian tube fimbriae can be the origin of cells for high-grade serous subtype of epithelial ovarian carcinoma (HGSOC). A mouse HGSOC model with conditional Dicer-Pten double knockout (Dicer-Pten DKO) developed primary tumors, intriguingly, from the fallopian tube stroma. We examined the growth and epithelial phenotypes of the Dicer-Pten DKO mouse tumor cells contributable by each gene knockout. Unlike human ovarian epithelial cancer cells that expressed full-length E-cadherin, the Dicer-Pten DKO stromal tumor cells expressed cleaved E-cadherin fragments and metalloproteinase 2, a mixture of epithelial and mesenchymal markers. Although the Dicer-Pten DKO tumor cells lost the expression of mature microRNAs as expected, they showed high levels of tRNA fragment expression and enhanced AKT activation due to the loss of PTEN function. Introduction of a Dicer1-expressing construct into the DKO mouse tumor cells significantly reduced DNA synthesis and the cell growth rate, with concurrent diminished adhesion and ZO1 epithelial staining. Hence, it is likely that the loss of Dicer promoted mesenchymal-epithelial transition in fallopian tube stromal cells, and in conjunction with Pten loss, further promoted cell proliferation and epithelial-like tumorigenesis.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherImpact Journals LLC
dc.relation.ispartofpagefrom66077
dc.relation.ispartofpageto66086
dc.relation.ispartofissue40
dc.relation.ispartofjournalOncotarget
dc.relation.ispartofvolume7
dc.subject.fieldofresearchOncology and carcinogenesis
dc.subject.fieldofresearchcode3211
dc.titleEpithelialization of mouse ovarian tumor cells originating in the fallopian tube stroma
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttps://creativecommons.org/licenses/by/3.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2016. This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorNg, Shu Kay Angus


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