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dc.contributor.authorKriger, Kerry
dc.contributor.authorB. Hines, Harry.
dc.contributor.authorD. Hyatt, Alex.
dc.contributor.authorG. Boyle, Donna.
dc.contributor.authorHero, Jean-Marc
dc.contributor.editorOtto Kinne
dc.date.accessioned2017-05-03T11:08:13Z
dc.date.available2017-05-03T11:08:13Z
dc.date.issued2006
dc.date.modified2010-08-04T02:36:49Z
dc.identifier.issn01775103
dc.identifier.doi10.3354/dao071141
dc.identifier.urihttp://hdl.handle.net/10072/13841
dc.description.abstractChytridiomycosis is a lethal disease of amphibians associated with mass mortalities and population declines worldwide. An accurate, non-invasive technique for detecting chytridiomycosis is urgently needed to determine the current geographical distribution of the disease, and its prevalence in wild amphibian populations. Herein we evaluate a recently devised, rapid, non-invasive, swab-PCR assay. We sampled 101 wild juvenile Mixophyes iteratus by both a skin swab for use in PCR analysis, and a toe-clip for examination by histological methods. The swab-PCR assay detected chytridiomycosis infection in a minimum of 14.9% of frogs, whereas histology detected infection in no more than 6.9% of frogs. We conclude that the swab-PCR technique is the more reliable means of detecting chytridiomycosis in wild amphibians, and that it precludes the need for toe-clipping as a means of sampling for the presence of the disease in future surveys. Further, we document a significant negative relationship between a juvenile frog's snout-vent length and its likelihood of being infected with the disease.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoen_AU
dc.publisherInter Research
dc.publisher.placeGermany
dc.relation.ispartofstudentpublicationY
dc.relation.ispartofpagefrom141
dc.relation.ispartofpageto148
dc.relation.ispartofedition2006
dc.relation.ispartofissue2
dc.relation.ispartofjournalDiseases of Aquatic Organisms
dc.relation.ispartofvolume71
dc.rights.retentionY
dc.subject.fieldofresearchHistory and Archaeology
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchAgricultural and Veterinary Sciences
dc.subject.fieldofresearchcode21
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode07
dc.titleTechniques for Detecting Chytridiomycosis in Wild Frogs: Comparing Histology with Real-Time Taqman PCR
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2006
gro.hasfulltextNo Full Text
gro.griffith.authorHero, Jean-Marc
gro.griffith.authorKriger, Kerry M.


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