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dc.contributor.authorL . Nielsen, Jeppeen_US
dc.contributor.authorKlausen, Cecileen_US
dc.contributor.authorNielsen, Peren_US
dc.contributor.authorBurford, Micheleen_US
dc.contributor.authorO. G Joergensen, Nielsen_US
dc.date.accessioned2017-05-03T14:13:43Z
dc.date.available2017-05-03T14:13:43Z
dc.date.issued2006en_US
dc.date.modified2008-11-21T03:20:24Z
dc.identifier.issn01686496en_US
dc.identifier.doi10.1111/j.1574-6941.2005.00054.xen_AU
dc.identifier.urihttp://hdl.handle.net/10072/13847
dc.description.abstractThe abundance, identity and activity of uncultured Bacteria and Actinobacteria present in a drinking water reservoir (North Pine Dam, Brisbane, Australia) were determined using a combination of fluorescence in situ hybridization (FISH) alone or with catalysed reporter deposition (CARD-FISH) with microautoradiography. The CARD-FISH technique was modified relative to previous described procedures and performed directly on gelatine cover slips in order to allow simultaneous combination with microautoradiography. Almost twofold higher numbers of microorganisms could be identified as either Bacteria or Actinobacteria using the CARD-FISH technique as compared with the traditional FISH technique. A combination of FISH or CARD-FISH with microautoradiography showed generally higher activity among the Actinobacteria than among all Bacteria. Another important observation was that many cells within the FISH-negative populations of both Actinobacteria and Bacteria were actively assimilating thymidine. Thus, great care should be taken when extrapolating the active fraction of a prokaryotic community to be equivalent to the FISH-detectable population in such environments. Bacterial groups within Actinobacteria produce the odours geosmin and 2-methylisoborneol, which lower the quality of surface water when used for drinking. The results indicate that combined microautoradiography and CARD-FISH may serve as an effective tool when studying identity and activity of microorganisms within freshwater environments.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherBlackwell Publishingen_US
dc.publisher.placeNetherlandsen_US
dc.publisher.urihttp://www3.interscience.wiley.com/journal/118503984/homeen_AU
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom432en_US
dc.relation.ispartofpageto438en_US
dc.relation.ispartofjournalFEMS Microbiology Ecologyen_US
dc.relation.ispartofvolume55en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchcode300801en_US
dc.titleDetection of activity among uncultured Actinobacteria in a drinking water reservoiren_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2006
gro.hasfulltextNo Full Text


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