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  • Pyocyanin inhibits both nitric oxide-dependent and -independent relaxation in porcine coronary arteries

    Author(s)
    Hempenstall, Allison
    Grant, Gary D
    Anoopkumar-Dukie, Shailendra
    Johnson, Peter J
    Griffith University Author(s)
    Grant, Gary D.
    Anoopkumar-Dukie, Shailendra
    Year published
    2015
    Metadata
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    Abstract
    The effects of the Pseudomonas aeruginosa virulence factor pyocyanin (PCN) on the contractile function of porcine coronary arteries was investigated in vitro. Artery rings (5 mm) were suspended in organ baths containing Krebs’ solution for the measurement of isometric tension. The effect of PCN on resting and precontracted coronary arteries was initially investigated with various agents. Arteries were precontracted with prostaglandin (PG) F2a or potassium chloride and endothelium- dependent relaxations were induced by various agents in the presence of PCN. Pyocyanin (0.1–10 lmol/L) evoked small-amplitude, dose-dependent ...
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    The effects of the Pseudomonas aeruginosa virulence factor pyocyanin (PCN) on the contractile function of porcine coronary arteries was investigated in vitro. Artery rings (5 mm) were suspended in organ baths containing Krebs’ solution for the measurement of isometric tension. The effect of PCN on resting and precontracted coronary arteries was initially investigated with various agents. Arteries were precontracted with prostaglandin (PG) F2a or potassium chloride and endothelium- dependent relaxations were induced by various agents in the presence of PCN. Pyocyanin (0.1–10 lmol/L) evoked small-amplitude, dose-dependent contractions in resting porcine coronary arteries. In addition, PCN amplified the contractile response to PGF2a, but did not alter responses to carbachol. Pyocyanin (0.1–10 lmol/L) significantly inhibited endothelium-dependent relaxations evoked by neurokinin A. Pyocyanin also inhibited relaxations evoked by diethylamine nitric oxide (a nitric oxide donor), forskolin (an adenylate cyclase activator), dibuytyryl-cAMP (a cAMP analogue), 8- bromo-cGMP (a cGMP analogue) and P1075 (a KATP channel activator), but not isoprenaline (b-adrenoceceptor agonist). These results indicate that physiological concentrations of PCN interfere with multiple intracellular processes involved in vascular smooth muscle relaxation, in particular pathways downstream of nitric oxide release. Thus, PCN may alter normal vascular function in patients infected with P. aeruginosa. Key words: coronary artery, nitric oxide, Pseudomonas aeruginosa, pyocyanin.
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    Journal Title
    Clinical and Experimental Pharmacology and Physiology
    Volume
    42
    Issue
    2
    DOI
    https://doi.org/10.1111/1440-1681.12340
    Subject
    Zoology
    Pharmacology and pharmaceutical sciences
    Medical physiology
    Medical physiology not elsewhere classified
    Publication URI
    http://hdl.handle.net/10072/140974
    Collection
    • Journal articles

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