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  • A Versatile Orthotopic Nude Mouse Model for Study of Esophageal Squamous Cell Carcinoma

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    Author(s)
    Ip, Joseph Chok Yan
    Ko, Josephine Mun Yee
    Yu, Valen Zhuoyou
    Chan, Kwok Wah
    Lam, Alfred K
    Law, Simon
    Tong, Daniel King Hung
    Lung, Maria Li
    Griffith University Author(s)
    Lam, Alfred K.
    Year published
    2015
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    Abstract
    Increasing evidence indicates tumor-stromal interactions play a crucial role in cancer. An in vivo esophageal squamous cell carcinoma (ESCC) orthotopic animal model was developed with bioluminescence imaging established with a real-time monitoring platform for functional and signaling investigation of tumor-stromal interactions. The model was produced by injection of luciferase-labelled ESCC cells into the intraesophageal wall of nude mice. Histological examination indicates this orthotopic model is highly reproducible with 100% tumorigenesis among the four ESCC cell lines tested. This new model recapitulates many clinical ...
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    Increasing evidence indicates tumor-stromal interactions play a crucial role in cancer. An in vivo esophageal squamous cell carcinoma (ESCC) orthotopic animal model was developed with bioluminescence imaging established with a real-time monitoring platform for functional and signaling investigation of tumor-stromal interactions. The model was produced by injection of luciferase-labelled ESCC cells into the intraesophageal wall of nude mice. Histological examination indicates this orthotopic model is highly reproducible with 100% tumorigenesis among the four ESCC cell lines tested. This new model recapitulates many clinical and pathological properties of human ESCC, including esophageal luminal stricture by squamous cell carcinoma with nodular tumor growth, adventitia invasion, lymphovascular invasion, and perineural infiltration. It was tested using an AKT shRNA knockdown of ESCC cell lines and the in vivo tumor suppressive effects of AKT knockdown were observed. In conclusion, this ESCC orthotopic mouse model allows investigation of gene functions of cancer cells in a more natural tumor microenvironment and has advantages over previous established models. It provides a versatile platform with potential application for metastasis and therapeutic regimen testing.
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    Journal Title
    BioMed Research International
    Volume
    2015
    DOI
    https://doi.org/10.1155/2015/910715
    Copyright Statement
    © 2015 Joseph Chok Yan Ip et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
    Subject
    Medical and Health Sciences not elsewhere classified
    Biological Sciences
    Information and Computing Sciences
    Technology
    Publication URI
    http://hdl.handle.net/10072/141165
    Collection
    • Journal articles

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