Show simple item record

dc.contributor.authorIp, Joseph Chok Yan
dc.contributor.authorKo, Josephine Mun Yee
dc.contributor.authorYu, Valen Zhuoyou
dc.contributor.authorChan, Kwok Wah
dc.contributor.authorLam, Alfred K
dc.contributor.authorLaw, Simon
dc.contributor.authorTong, Daniel King Hung
dc.contributor.authorLung, Maria Li
dc.date.accessioned2017-08-18T01:26:47Z
dc.date.available2017-08-18T01:26:47Z
dc.date.issued2015
dc.identifier.issn2314-6133
dc.identifier.doi10.1155/2015/910715
dc.identifier.urihttp://hdl.handle.net/10072/141165
dc.description.abstractIncreasing evidence indicates tumor-stromal interactions play a crucial role in cancer. An in vivo esophageal squamous cell carcinoma (ESCC) orthotopic animal model was developed with bioluminescence imaging established with a real-time monitoring platform for functional and signaling investigation of tumor-stromal interactions. The model was produced by injection of luciferase-labelled ESCC cells into the intraesophageal wall of nude mice. Histological examination indicates this orthotopic model is highly reproducible with 100% tumorigenesis among the four ESCC cell lines tested. This new model recapitulates many clinical and pathological properties of human ESCC, including esophageal luminal stricture by squamous cell carcinoma with nodular tumor growth, adventitia invasion, lymphovascular invasion, and perineural infiltration. It was tested using an AKT shRNA knockdown of ESCC cell lines and the in vivo tumor suppressive effects of AKT knockdown were observed. In conclusion, this ESCC orthotopic mouse model allows investigation of gene functions of cancer cells in a more natural tumor microenvironment and has advantages over previous established models. It provides a versatile platform with potential application for metastasis and therapeutic regimen testing.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherHindawi Publishing Corporation
dc.relation.ispartofpagefrom910715-1
dc.relation.ispartofpageto910715-10
dc.relation.ispartofjournalBioMed Research International
dc.relation.ispartofvolume2015
dc.subject.fieldofresearchMedical and Health Sciences not elsewhere classified
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchInformation and Computing Sciences
dc.subject.fieldofresearchTechnology
dc.subject.fieldofresearchcode119999
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode08
dc.subject.fieldofresearchcode10
dc.titleA Versatile Orthotopic Nude Mouse Model for Study of Esophageal Squamous Cell Carcinoma
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/3.0/
dc.description.versionPublished
gro.rights.copyright© 2015 Joseph Chok Yan Ip et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorLam, Alfred K.


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record