Evaluation of Respiration of Mitochondria in Cancer Cells Exposed to Mitochondria-Targeted Agents
Author(s)
Kluckova, K
Dong, LF
Bajzikova, M
Rohlena, J
Neuzil, J
Griffith University Author(s)
Year published
2015
Metadata
Show full item recordAbstract
Respiration is one of the major functions of mitochondria, whereby these vital organelles use oxygen to produce energy. Many agents that may be of potential clinical relevance act by targeting mitochondria, where they may suppress mitochondrial respiration. It is therefore important to evaluate this process and understand how this is modulated by small molecules. Here, we describe the general methodology to assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted to mitochondria on this process, and also how to assess this in tumor tissue.Respiration is one of the major functions of mitochondria, whereby these vital organelles use oxygen to produce energy. Many agents that may be of potential clinical relevance act by targeting mitochondria, where they may suppress mitochondrial respiration. It is therefore important to evaluate this process and understand how this is modulated by small molecules. Here, we describe the general methodology to assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted to mitochondria on this process, and also how to assess this in tumor tissue.
View less >
View less >
Book Title
Mitochondrial Medicine
Volume
2
Subject
Other chemical sciences
Biochemistry and cell biology
Biochemistry and cell biology not elsewhere classified