Pseudomonas aeruginosa genotypes acquired by children with cystic fibrosis by age 5-years
Author(s)
Kidd, Timothy J
Ramsay, Kay A
Vidmar, Suzanna
Carlin, John B
Bell, Scott C
Wainwright, Claire E
Grimwood, Keith
Wainwright, Claire E
Grimwood, Keith
Francis, Paul W
Dakin, Carolyn
Cheney, Joyce
George, Narelle
Carlin, John B
Robertson, Colin F
Vidmar, Suzanna
Moodie, Marj
Carzino, Rosemary
Carter, Robert
Armstrong, David S
Cooper, Peter J
Mckay, Karen
Martin, A James
Whitehead, Bruce
Hunter, John
By-Rnes, Catherine A
Tiddens, Harm A
Graniel, Karla
Gerbrands, Krista
Mott, Lauren
Griffith University Author(s)
Year published
2015
Metadata
Show full item recordAbstract
Background: We describe Pseudomonas aeruginosa acquisitions in children with cystic fibrosis (CF) aged ≤5-years, eradication treatment
efficacy, and genotypic relationships between upper and lower airway isolates and strains from non-CF sources.
Methods: Of 168 CF children aged ≤5-years in a bronchoalveolar lavage (BAL)-directed therapy trial, 155 had detailed microbiological results.
Overall, 201/271 (74%) P. aeruginosa isolates from BAL and oropharyngeal cultures were available for genotyping, including those collected
before and after eradication therapy.
Results: Eighty-two (53%) subjects acquired P. aeruginosa, of which ...
View more >Background: We describe Pseudomonas aeruginosa acquisitions in children with cystic fibrosis (CF) aged ≤5-years, eradication treatment efficacy, and genotypic relationships between upper and lower airway isolates and strains from non-CF sources. Methods: Of 168 CF children aged ≤5-years in a bronchoalveolar lavage (BAL)-directed therapy trial, 155 had detailed microbiological results. Overall, 201/271 (74%) P. aeruginosa isolates from BAL and oropharyngeal cultures were available for genotyping, including those collected before and after eradication therapy. Results: Eighty-two (53%) subjects acquired P. aeruginosa, of which most were unique strains. Initial eradication success rate was 90%, but 36 (44%) reacquired P. aeruginosa, with genotypic substitutions more common in BAL (12/14) than oropharyngeal (3/11) cultures. Moreover, oropharyngeal cultures did not predict BAL genotypes reliably. Conclusions: CF children acquire environmental P. aeruginosa strains frequently. However, discordance between BAL and oropharyngeal strains raises questions over upper airway reservoirs and how to best determine eradication in non-expectorating children.
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View more >Background: We describe Pseudomonas aeruginosa acquisitions in children with cystic fibrosis (CF) aged ≤5-years, eradication treatment efficacy, and genotypic relationships between upper and lower airway isolates and strains from non-CF sources. Methods: Of 168 CF children aged ≤5-years in a bronchoalveolar lavage (BAL)-directed therapy trial, 155 had detailed microbiological results. Overall, 201/271 (74%) P. aeruginosa isolates from BAL and oropharyngeal cultures were available for genotyping, including those collected before and after eradication therapy. Results: Eighty-two (53%) subjects acquired P. aeruginosa, of which most were unique strains. Initial eradication success rate was 90%, but 36 (44%) reacquired P. aeruginosa, with genotypic substitutions more common in BAL (12/14) than oropharyngeal (3/11) cultures. Moreover, oropharyngeal cultures did not predict BAL genotypes reliably. Conclusions: CF children acquire environmental P. aeruginosa strains frequently. However, discordance between BAL and oropharyngeal strains raises questions over upper airway reservoirs and how to best determine eradication in non-expectorating children.
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Journal Title
Journal of Cystic Fibrosis
Volume
14
Issue
3
Subject
Clinical Sciences not elsewhere classified
Clinical Sciences