High resolution melting curve analysis with MATLAB-based program
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Author(s)
Li, Huaizhong
Lan, Ruiting
Peng, Niancai
Sun, Jing
Zhu, Yong
Year published
2016
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High resolution melting curve analysis (HRM) is an emerging new method for interrogating and characterizing DNA samples. It has been used as a powerful tool for gene mutation and single-nucleotide polymorphism (SNP) detection with high throughput and low cost. Commercially available HRM analysis systems are mostly proprietary and expensive. It lacks the flexibility for the end users and researchers to incorporate new analysis algorithms into the existing system. This paper presents the development of a MATLAB-based open software program for high resolution melting curve analysis. Key analysis functions, such as to obtain the ...
View more >High resolution melting curve analysis (HRM) is an emerging new method for interrogating and characterizing DNA samples. It has been used as a powerful tool for gene mutation and single-nucleotide polymorphism (SNP) detection with high throughput and low cost. Commercially available HRM analysis systems are mostly proprietary and expensive. It lacks the flexibility for the end users and researchers to incorporate new analysis algorithms into the existing system. This paper presents the development of a MATLAB-based open software program for high resolution melting curve analysis. Key analysis functions, such as to obtain the first derivative curve using Savitzky–Golay filter, to identify the melt region, subtraction of background fluorescence and curve normalization, are introduced, followed by case studies of HRM analysis using the developed software program.
View less >
View more >High resolution melting curve analysis (HRM) is an emerging new method for interrogating and characterizing DNA samples. It has been used as a powerful tool for gene mutation and single-nucleotide polymorphism (SNP) detection with high throughput and low cost. Commercially available HRM analysis systems are mostly proprietary and expensive. It lacks the flexibility for the end users and researchers to incorporate new analysis algorithms into the existing system. This paper presents the development of a MATLAB-based open software program for high resolution melting curve analysis. Key analysis functions, such as to obtain the first derivative curve using Savitzky–Golay filter, to identify the melt region, subtraction of background fluorescence and curve normalization, are introduced, followed by case studies of HRM analysis using the developed software program.
View less >
Journal Title
Measurement
Volume
90
Copyright Statement
© 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
Subject
Artificial intelligence
Applied mathematics
Machining
Mechanical engineering
Information and computing sciences
Mathematical sciences