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  • Adaptation of the [3H]Hypoxanthine Uptake Assay for In Vitro-Cultured Plasmodium knowlesi Malaria Parasites

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    Author(s)
    Arnold, Megan SJ
    Engel, Jessica A
    Chua, Ming Jang
    Fisher, Gillian M
    Skinner-Adams, Tina S
    Andrews, Katherine T
    Griffith University Author(s)
    Andrews, Katherine T.
    Fisher, Gill M.
    Skinner-Adams, Tina
    Arnold, Megan
    Engel, Jess
    Chua, MJ J.
    Year published
    2016
    Metadata
    Show full item record
    Abstract
    The zoonotic malaria parasite Plasmodium knowlesi has recently been established in continuous in vitro culture. Here, the Plasmodium falciparum [3H]hypoxanthine uptake assay was adapted for P. knowlesi and used to determine the sensitivity of this parasite to chloroquine, cycloguanil, and clindamycin. The data demonstrate that P. knowlesi is sensitive to all drugs, with 50% inhibitory concentrations (IC50s) consistent with those obtained with P. falciparum. This assay provides a platform to use P. knowlesi in vitro for drug discovery.The zoonotic malaria parasite Plasmodium knowlesi has recently been established in continuous in vitro culture. Here, the Plasmodium falciparum [3H]hypoxanthine uptake assay was adapted for P. knowlesi and used to determine the sensitivity of this parasite to chloroquine, cycloguanil, and clindamycin. The data demonstrate that P. knowlesi is sensitive to all drugs, with 50% inhibitory concentrations (IC50s) consistent with those obtained with P. falciparum. This assay provides a platform to use P. knowlesi in vitro for drug discovery.
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    Journal Title
    Antimicrobial Agents and Chemotherapy
    Volume
    60
    Issue
    7
    DOI
    https://doi.org/10.1128/AAC.02948-15
    Copyright Statement
    © 2016 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
    Subject
    Medical Microbiology not elsewhere classified
    Microbiology
    Medical Microbiology
    Pharmacology and Pharmaceutical Sciences
    Publication URI
    http://hdl.handle.net/10072/142231
    Collection
    • Journal articles

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