dc.contributor.author | Arnold, Megan SJ | |
dc.contributor.author | Engel, Jessica A | |
dc.contributor.author | Chua, Ming Jang | |
dc.contributor.author | Fisher, Gillian M | |
dc.contributor.author | Skinner-Adams, Tina S | |
dc.contributor.author | Andrews, Katherine T | |
dc.date.accessioned | 2018-03-26T23:20:17Z | |
dc.date.available | 2018-03-26T23:20:17Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0066-4804 | |
dc.identifier.doi | 10.1128/AAC.02948-15 | |
dc.identifier.uri | http://hdl.handle.net/10072/142231 | |
dc.description.abstract | The zoonotic malaria parasite Plasmodium knowlesi has recently been established in continuous in vitro culture. Here, the Plasmodium falciparum [3H]hypoxanthine uptake assay was adapted for P. knowlesi and used to determine the sensitivity of this parasite to chloroquine, cycloguanil, and clindamycin. The data demonstrate that P. knowlesi is sensitive to all drugs, with 50% inhibitory concentrations (IC50s) consistent with those obtained with P. falciparum. This assay provides a platform to use P. knowlesi in vitro for drug discovery. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | American Society for Microbiology | |
dc.relation.ispartofpagefrom | 4361 | |
dc.relation.ispartofpageto | 4363 | |
dc.relation.ispartofissue | 7 | |
dc.relation.ispartofjournal | Antimicrobial Agents and Chemotherapy | |
dc.relation.ispartofvolume | 60 | |
dc.subject.fieldofresearch | Microbiology | |
dc.subject.fieldofresearch | Medical microbiology | |
dc.subject.fieldofresearch | Medical microbiology not elsewhere classified | |
dc.subject.fieldofresearch | Pharmacology and pharmaceutical sciences | |
dc.subject.fieldofresearchcode | 3107 | |
dc.subject.fieldofresearchcode | 3207 | |
dc.subject.fieldofresearchcode | 320799 | |
dc.subject.fieldofresearchcode | 3214 | |
dc.title | Adaptation of the [3H]Hypoxanthine Uptake Assay for In Vitro-Cultured Plasmodium knowlesi Malaria Parasites | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
dc.description.version | Version of Record (VoR) | |
gro.faculty | Griffith Sciences, School of Natural Sciences | |
gro.rights.copyright | © 2016 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Andrews, Katherine T. | |
gro.griffith.author | Fisher, Gill M. | |
gro.griffith.author | Skinner-Adams, Tina | |