Skin CD4+ memory T cells exhibit combined cluster-mediated retention and equilibration with the circulation

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Author(s)
Collins, Nicholas
Jiang, Xiaodong
Zaid, Ali
Macleod, Bethany L
Li, Jane
Park, Chang Ook
Haque, Ashraful
Bedoui, Sammy
Heath, William R
Mueller, Scott N
Kupper, Thomas S
Gebhardt, Thomas
Carbone, Francis R
Griffith University Author(s)
Year published
2016
Metadata
Show full item recordAbstract
Although memory T cells within barrier tissues can persist as permanent residents, at least
some exchange with blood. The extent to which this occurs is unclear. Here we show that
memory CD4þ T cells in mouse skin are in equilibrium with the circulation at steady state.
These cells are dispersed throughout the inter-follicular regions of the dermis and form
clusters with antigen presenting cells around hair follicles. After infection or administration of
a contact sensitizing agent, there is a sustained increase in skin CD4þ T-cell content, which
is confined to the clusters, with a concomitant CCL5-dependent increase in CD4þ ...
View more >Although memory T cells within barrier tissues can persist as permanent residents, at least some exchange with blood. The extent to which this occurs is unclear. Here we show that memory CD4þ T cells in mouse skin are in equilibrium with the circulation at steady state. These cells are dispersed throughout the inter-follicular regions of the dermis and form clusters with antigen presenting cells around hair follicles. After infection or administration of a contact sensitizing agent, there is a sustained increase in skin CD4þ T-cell content, which is confined to the clusters, with a concomitant CCL5-dependent increase in CD4þ T-cell recruitment. Skin CCL5 is derived from CD11bþ cells and CD8þ T cells, with the elimination of the latter decreasing CD4þ T-cell numbers. These results reveal a complex pattern of tissue-retention and equilibration for CD4þ memory T cells in skin, which is altered by infection and inflammation history.
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View more >Although memory T cells within barrier tissues can persist as permanent residents, at least some exchange with blood. The extent to which this occurs is unclear. Here we show that memory CD4þ T cells in mouse skin are in equilibrium with the circulation at steady state. These cells are dispersed throughout the inter-follicular regions of the dermis and form clusters with antigen presenting cells around hair follicles. After infection or administration of a contact sensitizing agent, there is a sustained increase in skin CD4þ T-cell content, which is confined to the clusters, with a concomitant CCL5-dependent increase in CD4þ T-cell recruitment. Skin CCL5 is derived from CD11bþ cells and CD8þ T cells, with the elimination of the latter decreasing CD4þ T-cell numbers. These results reveal a complex pattern of tissue-retention and equilibration for CD4þ memory T cells in skin, which is altered by infection and inflammation history.
View less >
Journal Title
Nature Communications
Volume
7
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Subject
Cellular Immunology