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dc.contributor.authorHeupink, Tim H
dc.contributor.authorSubramanian, Sankar
dc.contributor.authorWright, Joanne L
dc.contributor.authorEndicott, Phillip
dc.contributor.authorWestaway, Michael Carrington
dc.contributor.authorHuynen, Leon
dc.contributor.authorParson, Walther
dc.contributor.authorMillar, Craig D
dc.contributor.authorWillerslev, Eske
dc.contributor.authorLambert, David M
dc.date.accessioned2018-01-23T01:01:07Z
dc.date.available2018-01-23T01:01:07Z
dc.date.issued2016
dc.identifier.issn0027-8424
dc.identifier.doi10.1073/pnas.1521066113
dc.identifier.urihttp://hdl.handle.net/10072/142553
dc.description.abstractThe publication in 2001 by Adcock et al. [Adcock GJ, et al. (2001) Proc Natl Acad Sci USA 98(2):537–542] in PNAS reported the recovery of short mtDNA sequences from ancient Australians, including the 42,000-y-old Mungo Man [Willandra Lakes Hominid (WLH3)]. This landmark study in human ancient DNA suggested that an early modern human mitochondrial lineage emerged in Asia and that the theory of modern human origins could no longer be considered solely through the lens of the “Out of Africa” model. To evaluate these claims, we used second generation DNA sequencing and capture methods as well as PCR-based and single-primer extension (SPEX) approaches to reexamine the same four Willandra Lakes and Kow Swamp 8 (KS8) remains studied in the work by Adcock et al. Two of the remains sampled contained no identifiable human DNA (WLH15 and WLH55), whereas the Mungo Man (WLH3) sample contained no Aboriginal Australian DNA. KS8 reveals human mitochondrial sequences that differ from the previously inferred sequence. Instead, we recover a total of five modern European contaminants from Mungo Man (WLH3). We show that the remaining sample (WLH4) contains ∼1.4% human DNA, from which we assembled two complete mitochondrial genomes. One of these was a previously unidentified Aboriginal Australian haplotype belonging to haplogroup S2 that we sequenced to a high coverage. The other was a contaminating modern European mitochondrial haplotype. Although none of the sequences that we recovered matched those reported by Adcock et al., except a contaminant, these findings show the feasibility of obtaining important information from ancient Aboriginal Australian remains.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNational Academy of Sciences
dc.relation.ispartofpagefrom6892
dc.relation.ispartofpageto6897
dc.relation.ispartofissue25
dc.relation.ispartofjournalProceedings of the National Academy of Sciences of the United States of America
dc.relation.ispartofvolume113
dc.subject.fieldofresearchEvolutionary Biology not elsewhere classified
dc.subject.fieldofresearchcode060399
dc.titleAncient mtDNA sequences from the First Australians revisited
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Sciences, Griffith School of Environment
gro.hasfulltextNo Full Text
gro.griffith.authorWright, Joanne L.
gro.griffith.authorLambert, David M.
gro.griffith.authorSankarasubramanian, Sankar
gro.griffith.authorHeupink, Tim H.
gro.griffith.authorHuynen, Leon
gro.griffith.authorWestaway, Michael
gro.griffith.authorWillerslev, Eske


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