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  • Defence gene expression profiling to Ascochyta rabiei aggressiveness in chickpea

    Author(s)
    Leo, Audrey E
    Linde, Celeste C
    Ford, Rebecca
    Griffith University Author(s)
    Ford, Rebecca
    Year published
    2016
    Metadata
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    Abstract
    A high number of Ascochyta rabiei pathotypes infecting chickpea in Australia has severely hampered efforts towards breeding for sustained quantitative resistance in chickpea. Breeding for sustained resistance will be aided by detailed knowledge of defence responses to isolates with different aggressiveness. As an initial step, the conserved and differential expressions of a suit of previously characterised genes known to be involved in fungal defence mechanisms were assessed among resistant and susceptible host genotypes following inoculation with high or low aggressive A. rabiei isolates. Using quantitative Real-Time PCR ...
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    A high number of Ascochyta rabiei pathotypes infecting chickpea in Australia has severely hampered efforts towards breeding for sustained quantitative resistance in chickpea. Breeding for sustained resistance will be aided by detailed knowledge of defence responses to isolates with different aggressiveness. As an initial step, the conserved and differential expressions of a suit of previously characterised genes known to be involved in fungal defence mechanisms were assessed among resistant and susceptible host genotypes following inoculation with high or low aggressive A. rabiei isolates. Using quantitative Real-Time PCR (qRT-PCR), 15 defence-related genes, normalised with two reference genes, were temporally differentially expressed (P < 0.005) as early as 2 h post inoculation of Genesis090 (resistant) or Kaniva (susceptible). The highly aggressive isolate, 09KAL09, induced vastly different expression profiles of eight key defence-related genes among resistant and susceptible genotypes. Six of these same genes were differentially expressed among ten host genotypes, inclusive of the best resistance sources within the Australian chickpea breeding program, indicating potential use for discrimination and selection of resistance “type” in future breeding pursuits.
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    Journal Title
    Theoretical and Applied Genetics
    Volume
    129
    Issue
    7
    DOI
    https://doi.org/10.1007/s00122-016-2706-2
    Subject
    Biological sciences
    Gene expression (incl. microarray and other genome-wide approaches)
    Agricultural, veterinary and food sciences
    Publication URI
    http://hdl.handle.net/10072/142647
    Collection
    • Journal articles

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