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dc.contributor.authorChacko, Anu
dc.contributor.authorStaines, Donald R
dc.contributor.authorJohnston, Samantha C
dc.contributor.authorMarshall-Gradisnik, Sonya M
dc.date.accessioned2018-01-22T06:43:46Z
dc.date.available2018-01-22T06:43:46Z
dc.date.issued2016
dc.identifier.issn1177-6250
dc.identifier.doi10.4137/GRSB.S40036
dc.identifier.urihttp://hdl.handle.net/10072/142888
dc.description.abstractBackground The etiology and pathomechanism of chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) are unknown. However, natural killer (NK) cell dysfunction, in particular reduced NK cytotoxic activity, is a consistent finding in CFS/ME patients. Previous research has reported significant changes in intracellular mitogen-activated protein kinase pathways from isolated NK cells. The purpose of this present investigation was to examine whether protein kinase genes have a role in abnormal NK cell intracellular signaling in CFS/ME. Method Messenger RNA (mRNA) expression of 528 protein kinase genes in isolated NK cells was analyzed (nCounter GX Human Kinase Kit v2 (XT); NanoString Technologies) from moderate (n = 11; age, 54.9 ± 10.3 years) and severe (n = 12; age, 47.5 ± 8.0 years) CFS/ME patients (classified by the 2011 International Consensus Criteria) and nonfatigued controls (n = 11; age, 50.0 ± 12.3 years). Results The expression of 92 protein kinase genes was significantly different in the severe CFS/ME group compared with nonfatigued controls. Among these, 37 genes were significantly upregulated and 55 genes were significantly downregulated in severe CFS/ME patients compared with nonfatigued controls. Conclusions In severe CFS/ME patients, dysfunction in protein kinase genes may contribute to impairments in NK cell intracellular signaling and effector function. Similar changes in protein kinase genes may be present in other cells, potentially contributing to the pathomechanism of this illness.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherLibertas Academica
dc.relation.ispartofpagefrom85
dc.relation.ispartofpageto93
dc.relation.ispartofjournalGene Regulation and Systems Biology
dc.relation.ispartofvolume10
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchGenetics not elsewhere classified
dc.subject.fieldofresearchcode3105
dc.subject.fieldofresearchcode310599
dc.titleDysregulation of Protein Kinase Gene Expression in NK Cells from Chronic Fatigue Syndrome/Myalgic Encephalomyelitis Patients
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttps://creativecommons.org/licenses/by-nc/3.0/
dc.description.versionVersion of Record (VoR)
gro.facultyGriffith Health, Menzies Health Institute
gro.rights.copyright© 2016 This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
gro.hasfulltextFull Text
gro.griffith.authorStaines, Donald R.
gro.griffith.authorMarshall-Gradisnik, Sonya M.


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