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dc.contributor.authorVrahnas, Christina
dc.contributor.authorPearson, Thomas A
dc.contributor.authorBrunt, Athena R
dc.contributor.authorForwood, Mark R
dc.contributor.authorBambery, Keith R
dc.contributor.authorTobin, Mark J
dc.contributor.authorMartin, T John
dc.contributor.authorSims, Natalie A
dc.date.accessioned2019-03-29T03:41:50Z
dc.date.available2019-03-29T03:41:50Z
dc.date.issued2016
dc.identifier.issn8756-3282
dc.identifier.doi10.1016/j.bone.2016.09.022
dc.identifier.urihttp://hdl.handle.net/10072/143043
dc.description.abstractIntermittent administration of parathyroid hormone (PTH) is used to stimulate bone formation in patients with osteoporosis. A reduction in the degree of matrix mineralisation has been reported during treatment, which may reflect either production of undermineralised matrix or a greater proportion of new matrix within the bone samples assessed. To explore these alternatives, high resolution synchrotron-based Fourier Transform Infrared Microspectroscopy (sFTIRM) coupled with calcein labelling was used in a region of non-remodelling cortical bone to determine bone composition during anabolic PTH treatment compared with region-matched samples from controls. 8 week old male C57BL/6 mice were treated with vehicle or 50 μg/kg PTH, 5 times/week for 4 weeks (n = 7–9/group). Histomorphometry confirmed greater trabecular and periosteal bone formation and 3-point bending tests confirmed greater femoral strength in PTH-treated mice. Dual calcein labels were used to match bone regions by time-since-mineralisation (bone age) and composition was measured by sFTIRM in six 15 μm2 regions at increasing depth perpendicular to the most immature bone on the medial periosteal edge; this allowed in situ measurement of progressive changes in bone matrix during its maturation. The sFTIRM method was validated in vehicle-treated bones where the expected progressive increases in mineral:matrix ratio and collagen crosslink type ratio were detected with increasing bone maturity. We also observed a gradual increase in carbonate content that strongly correlated with an increase in longitudinal stretch of the collagen triple helix (amide I:amide II ratio). PTH treatment did not alter the progressive changes in any of these parameters from the periosteal edge through to the more mature bone. These data provide new information about how the bone matrix matures in situ and confirm that bone deposited during PTH treatment undergoes normal collagen maturation and normal mineral accrual.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom146
dc.relation.ispartofpageto154
dc.relation.ispartofjournalBone
dc.relation.ispartofvolume93
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchEngineering
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode40
dc.subject.fieldofresearchcode32
dc.titleAnabolic action of parathyroid hormone (PTH) does not compromise bone matrix mineral composition or maturation
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyright© 2016 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorForwood, Mark R.


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