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dc.contributor.authorZulfiker, Abu Hasanat Md
dc.contributor.authorHashimi, Saeed M
dc.contributor.authorQi, Ji
dc.contributor.authorGrice, I Darren
dc.contributor.authorWei, Ming Q
dc.date.accessioned2017-12-15T06:01:53Z
dc.date.available2017-12-15T06:01:53Z
dc.date.issued2016
dc.identifier.issn0730-2312
dc.identifier.doi10.1002/jcb.25577
dc.identifier.urihttp://hdl.handle.net/10072/143059
dc.description.abstractToad skin extracts, such as aqueous extracts (AE) of Chinese toad skins, have demonstrated therapeutic benefits for a range of diseases including pain, inflammation, swelling, heart failure, and various types of cancers. In this study, we investigated the anti-inflammatory potential of an AE (0.1–10 μg/mL) and a 60% ethanol extract (EE; 0.1–10 μg/mL) from Australian cane toad (Bufo marinus) skins and the known bioactive compound, bufotenine (BT; 0.1–10 nM). The assay employed a model of the human monocyte cell line U937 stimulated with lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate (PMA) for the release of tumor necrosis factor (TNF)-α and interleukin (IL)-6. We demonstrated that AE, EE, and BT significantly inhibited the release and expression of TNF-α and IL-6 in a dose-dependent manner when the cells were pre-treated at non-cytotoxic concentrations. Further investigation revealed that the inhibition of TNF-α and IL-6 release and expression was associated with the suppression of nuclear factor (NF)-kappa (κ)B activation. These results indicate that AE, EE, and BT are strong inflammation inhibitors, thus have the potential for further development as anti-inflammatory therapeutic agents from a natural source regarded as a feral pest in Australia.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherJohn Wiley & Sons
dc.relation.ispartofpagefrom2769
dc.relation.ispartofpageto2780
dc.relation.ispartofissue12
dc.relation.ispartofjournalJournal of Cellular Biochemistry
dc.relation.ispartofvolume117
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchBiochemistry and cell biology not elsewhere classified
dc.subject.fieldofresearchMedical physiology
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode310199
dc.subject.fieldofresearchcode3208
dc.titleAqueous and Ethanol Extracts of Australian Cane Toad Skins Suppress Pro-Inflammatory Cytokine Secretion in U937 Cells via NF-κB Signaling Pathway
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medical Science
gro.hasfulltextNo Full Text
gro.griffith.authorGrice, Darren D.
gro.griffith.authorWei, Ming Q.


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