dc.contributor.author | Zulfiker, Abu Hasanat Md | |
dc.contributor.author | Hashimi, Saeed M | |
dc.contributor.author | Qi, Ji | |
dc.contributor.author | Grice, I Darren | |
dc.contributor.author | Wei, Ming Q | |
dc.date.accessioned | 2017-12-15T06:01:53Z | |
dc.date.available | 2017-12-15T06:01:53Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0730-2312 | |
dc.identifier.doi | 10.1002/jcb.25577 | |
dc.identifier.uri | http://hdl.handle.net/10072/143059 | |
dc.description.abstract | Toad skin extracts, such as aqueous extracts (AE) of Chinese toad skins, have demonstrated therapeutic benefits for a range of diseases including pain, inflammation, swelling, heart failure, and various types of cancers. In this study, we investigated the anti-inflammatory potential of an AE (0.1–10 μg/mL) and a 60% ethanol extract (EE; 0.1–10 μg/mL) from Australian cane toad (Bufo marinus) skins and the known bioactive compound, bufotenine (BT; 0.1–10 nM). The assay employed a model of the human monocyte cell line U937 stimulated with lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate (PMA) for the release of tumor necrosis factor (TNF)-α and interleukin (IL)-6. We demonstrated that AE, EE, and BT significantly inhibited the release and expression of TNF-α and IL-6 in a dose-dependent manner when the cells were pre-treated at non-cytotoxic concentrations. Further investigation revealed that the inhibition of TNF-α and IL-6 release and expression was associated with the suppression of nuclear factor (NF)-kappa (κ)B activation. These results indicate that AE, EE, and BT are strong inflammation inhibitors, thus have the potential for further development as anti-inflammatory therapeutic agents from a natural source regarded as a feral pest in Australia. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.publisher | John Wiley & Sons | |
dc.relation.ispartofpagefrom | 2769 | |
dc.relation.ispartofpageto | 2780 | |
dc.relation.ispartofissue | 12 | |
dc.relation.ispartofjournal | Journal of Cellular Biochemistry | |
dc.relation.ispartofvolume | 117 | |
dc.subject.fieldofresearch | Biochemistry and cell biology | |
dc.subject.fieldofresearch | Biochemistry and cell biology not elsewhere classified | |
dc.subject.fieldofresearch | Medical physiology | |
dc.subject.fieldofresearchcode | 3101 | |
dc.subject.fieldofresearchcode | 310199 | |
dc.subject.fieldofresearchcode | 3208 | |
dc.title | Aqueous and Ethanol Extracts of Australian Cane Toad Skins Suppress Pro-Inflammatory Cytokine Secretion in U937 Cells via NF-κB Signaling Pathway | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.faculty | Griffith Health, School of Medical Science | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Grice, Darren D. | |
gro.griffith.author | Wei, Ming Q. | |