dc.contributor.author | Lee, Ryan SB | |
dc.contributor.author | Hamlet, Stephen M | |
dc.contributor.author | Ivanovski, Saso | |
dc.date.accessioned | 2018-10-11T00:35:24Z | |
dc.date.available | 2018-10-11T00:35:24Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0905-7161 | |
dc.identifier.doi | 10.1111/clr.12979 | |
dc.identifier.uri | http://hdl.handle.net/10072/143100 | |
dc.description.abstract | Objectives: This study assessed the effect of titanium surface modification on macrophage
phenotype polarization and osseous healing under diabetic conditions.
Materials and methods: Critical-sized calvarial defects were created in healthy and streptozotocininduced
type I diabetic Sprague-Dawley rats. Titanium (Ti) discs with either large-grit sandblasted
and acid-etched micro-rough (SLA) or hydrophilic-modified SLA (modSLA) surfaces were used to
cover the healing defect for a period of up to 28 days. Samples of the exudate within the calvarial
defect and beneath the titanium discs were collected 1, 4 and 7 days post-surgery for inflammatory
cytokine analysis using an ELISA. The macrophage phenotype(s) on the Ti disc surfaces were
determined by CD11c+ (M1) and CD163+ (M2) immunofluorescent staining. Samples of the healing
defects at days 14 and 28 were also prepared for histomorphometric analysis.
Results: Cytokine levels in the diabetic animals were higher than those of the healthy group
throughout the observation period. The modSLA surface significantly reduced MIP-2 levels at day 1 in
both diabetic and healthy animals, and MCP-1 levels at day 4 in the diabetic animals. Immunofluorescent
staining showed that an M2-like macrophage phenotype was more frequently found on
the modSLA surface at day 1 in healthy and day 4 in both healthy and diabetic animals.
Histomorphometric analysis showed more new bone formation on the modSLA surface at days 14 and
28 in both groups, although statistically significant differences were only found in the healthy group.
Conclusion: Diabetic conditions greatly increased the expression of proinflammatory cytokines
during osseous healing. The modSLA surface was shown to promote an M2-like macrophage
phenotypic response in titanium adherent macrophages despite the significantly elevated
inflammatory environment induced by uncontrolled type I diabetes. Modulation of the
macrophage phenotype by the modSLA surface in the early healing period was associated with
osseous healing under both healthy and uncontrolled diabetic conditions. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Wiley-Blackwell Publishing | |
dc.relation.ispartofpagefrom | 1 | |
dc.relation.ispartofpageto | 10 | |
dc.relation.ispartofjournal | Clinical Oral Implants Research | |
dc.subject.fieldofresearch | Biomedical engineering | |
dc.subject.fieldofresearch | Dentistry | |
dc.subject.fieldofresearch | Dentistry not elsewhere classified | |
dc.subject.fieldofresearch | Clinical sciences | |
dc.subject.fieldofresearchcode | 4003 | |
dc.subject.fieldofresearchcode | 3203 | |
dc.subject.fieldofresearchcode | 320399 | |
dc.subject.fieldofresearchcode | 3202 | |
dc.title | The influence of titanium surface characteristics on macrophage phenotype polarization during osseous healing in type I diabetic rats: a pilot study | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.faculty | Griffith Health, School of Dentistry and Oral Health | |
gro.description.notepublic | This publication has been entered into Griffith Research Online as an Advanced Online Version. | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Hamlet, Stephen | |